000			02838nab a22004217a 4500
001			G93445
003			MX-TxCIM
005			20230804205719.0
008			210804t2009 xxk|||p|op||| 00| 0 eng d
022			_a1439-0523 (Online)
022			_a0179-9541
024	8		_ahttps://doi.org/10.1111/j.1439-0523.2008.01618.x
040			_aMX-TxCIM
041			_aeng
090			_aCIS-5626
100	1		_aXu, X. _921840
245	1	2	_aA high-throughput, low cost gel-based SNP assay for positional cloning and marker assisted breeding of useful genes in cereals
260			_aUnited Kingdom : _bWiley, _c2009.
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0179-9541
520			_aSeveral SNP (single nucleotide polymorphism) genotyping methods have been developed in the past most of which require sophisticated instrumentation and large initial investments. We describe here a high-throughput SSCP (single strand conformation polymorphism) system on our HEGS (high efficiency genome scanning) platform, which is simple, accurate, cost effective and requires neither restriction digestion of the amplification products nor elaborate post-PCR processing detection. Several parameters critical to SSCP analysis were optimized viz., gel matrix and concentration, gel running temperature, buffer composition, running conditions and PCR primer design so as to identify SNPs in amplicons ranging from 100 to 750 bp in size. A simple post-PCR processing system was developed using fluorescent dye for quick and easy detection of SNP polymorphism. HEGS-SSCP was also found to be useful in uncovering simple sequence repeat differences between different genotypes that differ by one or few di/tri nucleotide repeats. The practical utility of this system is illustrated with two successful efforts towards construction of high resolution linkage maps of a lesion mimic locus on chromosome 7 and a major quantitative trait locus conditioning field blast resistance on chromosome 4 in rice.
536			_aGlobal Maize Program
546			_aText in English
591			_aJohn Wiley
594			_aINT2925
650		7	_aSingle nucleotide polymorphisms _2AGROVOC _910805
650		7	_aPCR _2AGROVOC _912563
650		7	_aGenomics _2AGROVOC _91132
650		7	_aMarker-assisted selection _2AGROVOC _910737
650		7	_aGenetic markers _2AGROVOC _91848
650		7	_aCereals _2AGROVOC _91036
700	1		_9875 _aBABU, R. _gGlobal Maize Program _8INT2925
700	1		_921841 _aFujimura, T.
700	0		_94170 _aShinji Kawasaki
773	0		_tPlant Breeding _gv. 128, no. 4, p. 325-331 _dUnited Kingdom : Wiley, 2009. _wG445212 _x1439-0523
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/337
942			_cJA _2ddc _n0
999			_c27853 _d27853