| 000 | 03907nab a22004217a 4500 | ||
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| 001 | G93430 | ||
| 003 | MX-TxCIM | ||
| 005 | 20220523225447.0 | ||
| 008 | 220523s2009 xxk|||p|op||| 00| 0 eng d | ||
| 022 | _a0018-0661 | ||
| 022 | _a1601-5223 (Online) | ||
| 024 | 8 | _ahttps://doi.org/10.1111/j.1601-5223.2009.02132.x | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 090 | _aCIS-5610 | ||
| 100 | 1 |
_9842 _aJiankang Wang _gGenetic Resources Program _8INT2542 |
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| 245 | 1 | 0 | _aCloning and phylogenetic analysis of phytoene synthase 1 (Psy1) genes in common wheat and related species |
| 260 |
_aUnited Kingdom : _bWiley, _c2009. |
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| 500 | _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0018-0661 | ||
| 500 | _aPeer review | ||
| 500 | _aOpen Access | ||
| 520 | _aCloning and phylogenetic analysis of Psy1 genes in common wheat and its relatives would help to understand the genetic diversity and evolution of Psy1 gene in common wheat and its related species. In the present study, common wheat (AABBDD) and eight relative species, including T. urartu (AuAu), T. boeoticum (AmAm), T. monococcum (AmAm), Ae. speltoides (SS), Ae. tauschii (DD), T. dicoccoides (AABB), T. dicoccum (AABB) and T. spelta (AABBDD), were sampled for the isolation of novel alleles at Psy1-A1, Psy1-B1/Psy1-S1 and Psy1-D1 loci corresponding to common wheat Psy1 genes, and 27 new alleles were identified at these loci, designated Psy1-A1f through Psy1-A1k, Psy1-A1m and Psy1-A1n, Psy1-B1h through Psy1-B1m, Psy1-S1a through Psy1-S1c, Psy1-D1a through Psy1-D1j, respectively. The genes contained six exons and five introns, and the sequences of exons were more conserved compared with those of introns. The Psy1-A1 genes encoded a polypeptide of 428 aminoacid residues, with one residue longer than those encoded by Psy1-D1 genes. The Psy1-B1/Psy1-S1 genes encoded four types of polypeptides, with 421 (Psy1-B1h through Psy1-B1j, Psy1-B1l), 427 (Psy1-B1k, Psy1-S1a and Psy1-S1c), 428 (Psy1-B1m), and 429 (Psy1-S1b) aminoacid residues, respectively. Neighbor joining tree was generated based on the gene sequences of the 27 novel alleles and those of the 13 alleles reported previously in common wheat and its relatives. The phylogenetic tree consisted of two subtrees. The subtree I comprised 11 of 14 alleles at Psy1-A1 locus, nine of 16 alleles at Psy1-B1/Psy1-S1 locus, and ten novel alleles at Psy1-D1 locus, while the subtree II included the other three alleles at Psy1-A1 locus, the remaining four Psy1-B1 alleles and three Psy1-S1 alleles. The alleles from different clusters showed high sequence divergences, indicated by various SNPs and InDels (insertion/deletion). The phylogenetic relationships of these allelic variants at the three loci in common wheat and its relatives also supported the hypothesis that common wheat was originated by recurrent hexaploidization events. In addition, 193 Chinese wheat cultivars with different yellow pigment contents were genotyped with two novel STS markers YP7D-1 and YP7D-2. The results indicated that 191 cultivars contained the allele Psy1-D1a, and two had Psy1-D1g. | ||
| 536 | _aGenetic Resources Program|Global Wheat Program | ||
| 546 | _aText in English | ||
| 594 | _aINT2411|INT3297|INT2411|INT2542 | ||
| 650 | 7 |
_aWheat _2AGROVOC _91310 |
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| 650 | 7 |
_95948 _aCloning _2AGROVOC |
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| 650 | 7 |
_99129 _aPhylogeny _2AGROVOC |
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| 650 | 7 |
_91937 _aNucleotide sequence _2AGROVOC |
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| 650 | 7 |
_92185 _aAlleles _2AGROVOC |
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| 700 | 1 |
_9913 _aXinyao He _gGlobal Wheat Program _8INT3297 |
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| 700 | 1 |
_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838 |
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| 700 | 0 |
_aHui Wang _94189 |
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| 700 | 1 |
_9377 _aXianchun Xia |
|
| 773 | 0 |
_tHereditas _gv. 146, no. 5, p. 208-256 _dUnited Kingdom : Wiley, 2009. _wG444334 _x1601-5223 |
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| 856 | 4 |
_yOpen Access through DSpace _uhttp://hdl.handle.net/10883/1776 |
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| 942 |
_cJA _2ddc _n0 |
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| 999 |
_c27841 _d27841 |
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