000 | 02492nab a22004697a 4500 | ||
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001 | G93423 | ||
003 | MX-TxCIM | ||
005 | 20230804220328.0 | ||
008 | 210930s2009 xxu|||p|op||| 00| 0 eng d | ||
022 | _a0733-5210 | ||
024 | 8 | _ahttps://doi.org/10.1016/j.jcs.2009.05.006 | |
040 | _aMX-TxCIM | ||
041 | _aeng | ||
090 | _aCIS-5603 | ||
100 | 1 |
_aLiu, L. _9573 |
|
245 | 1 | 2 | _aA MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding |
260 |
_aUSA : _bElsevier, _c2009. |
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500 | _aPeer review | ||
500 | _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0733-5210 | ||
520 | _aHigh molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7OE, 7OE+8, 7OE+8a*, 7OE+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7OE, 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki. | ||
536 | _aGlobal Wheat Program | ||
546 | _aText in English | ||
591 | _aElsevier | ||
594 | _aINT2411 | ||
650 | 7 |
_aTriticum aestivum _2AGROVOC _91296 |
|
650 | 7 |
_aQuality _2AGROVOC _91231 |
|
650 | 7 |
_aGel electrophoresis _2AGROVOC _922497 |
|
650 | 7 |
_aMass spectrometry _2AGROVOC _911789 |
|
650 | 7 |
_aGlutenins _2AGROVOC _91137 |
|
700 | 0 |
_920799 _aAili Wang |
|
700 | 1 |
_91980 _aAppels, R. |
|
700 | 0 |
_923475 _aJunhong Ma |
|
700 | 0 |
_9377 _aXianchun Xia |
|
700 | 0 |
_923476 _aPing Lan |
|
700 | 1 |
_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838 |
|
700 | 1 |
_920838 _aBekes, F. |
|
700 | 0 |
_915752 _aYueming Yan |
|
700 | 0 |
_91979 _aWujun Ma |
|
773 | 0 |
_tJournal of Cereal Science _gv. 50, no. 2, p. 295-301 _dUSA : Elsevier, 2009. _wG444514 _x0733-5210 |
|
856 | 4 |
_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/1436 |
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942 |
_cJA _2ddc _n0 |
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999 |
_c27834 _d27834 |