000 02492nab a22004697a 4500
001 G93423
003 MX-TxCIM
005 20230804220328.0
008 210930s2009 xxu|||p|op||| 00| 0 eng d
022 _a0733-5210
024 8 _ahttps://doi.org/10.1016/j.jcs.2009.05.006
040 _aMX-TxCIM
041 _aeng
090 _aCIS-5603
100 1 _aLiu, L.
_9573
245 1 2 _aA MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding
260 _aUSA :
_bElsevier,
_c2009.
500 _aPeer review
500 _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0733-5210
520 _aHigh molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7OE, 7OE+8, 7OE+8a*, 7OE+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7OE, 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki.
536 _aGlobal Wheat Program
546 _aText in English
591 _aElsevier
594 _aINT2411
650 7 _aTriticum aestivum
_2AGROVOC
_91296
650 7 _aQuality
_2AGROVOC
_91231
650 7 _aGel electrophoresis
_2AGROVOC
_922497
650 7 _aMass spectrometry
_2AGROVOC
_911789
650 7 _aGlutenins
_2AGROVOC
_91137
700 0 _920799
_aAili Wang
700 1 _91980
_aAppels, R.
700 0 _923475
_aJunhong Ma
700 0 _9377
_aXianchun Xia
700 0 _923476
_aPing Lan
700 1 _aHe Zhonghu
_gGlobal Wheat Program
_8INT2411
_9838
700 1 _920838
_aBekes, F.
700 0 _915752
_aYueming Yan
700 0 _91979
_aWujun Ma
773 0 _tJournal of Cereal Science
_gv. 50, no. 2, p. 295-301
_dUSA : Elsevier, 2009.
_wG444514
_x0733-5210
856 4 _yAccess only for CIMMYT Staff
_uhttps://hdl.handle.net/20.500.12665/1436
942 _cJA
_2ddc
_n0
999 _c27834
_d27834