000			04410nab a22004817a 4500
001			G93418
003			MX-TxCIM
005			20230818214016.0
008			210705s2010 ne |||p|op||| 00| 0 eng d
022			_a1573-5060 (Online)
022			_a0014-2336
024	8		_ahttps://doi.org/10.1007/s10681-009-0054-x
040			_aMX-TxCIM
041			_aeng
090			_aCIS-5848
100	0		_aDan Liang _920831
245	1	0	_aCharacterization of CIMMYT bread wheats for high- and low-molecular weight glutenin subunits and other quality-related genes with SDS-PAGE, RP-HPLC and molecular markers
260			_aDordrecht (Netherlands) : _bSpringer, _c2010.
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0014-2336
520			_aTwo hundred and seventy-three CIMMYT bread wheat cultivars and advanced lines grown under irrigated conditions in Mexico during the 2005-06 Yaqui crop cycle were characterized for quality-related genetic traits using gene-specific markers for some high- and low-molecular-weight glutenin subunit (HMW-GS and LMW-GS) genes, polyphenol oxidase (PPO), phytoene synthase (PSY), and waxy genes. Of them, 142 were analyzed for quality parameters including SDS sedimentation volume (SDS-SV), dough mixing time, and Alveograph parameters, and for HMW-GS and LMW-GS compositions using sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and reversed-phase high-performance liquid chromatography (RP-HPLC). For the Ppo-A1 locus tested with the marker PPO18, the frequencies of alleles Ppo-A1a and Ppo-A1b were 79.1 and 20.2%, respectively, and no PCR fragment was amplified in 2 lines (0.73%), whereas 227 lines (83.2%) contained the allele Ppo-D1a and 46 lines (16.8%) had Ppo-D1b detected by markers PPO16 and PPO29. For the marker YP7A, 142 lines (52.0%) were assumed to have the allele Psy-A1a and 131 lines (48.0%) contained the allele Psy-A1b. In the case of the marker YP7B for the gene Psy-B1, the alleles Psy-B1a and Psy-B1b were detected in 155 (56.8%) and 43 (15.8%) lines, respectively, and 75 (27.4%) lines possessed the allele Psy-B1d detected by the marker YP7B-3. All 273 lines contained the alleles Wx-A1a and Wx-D1a as determined by markers MAG264 and MAG269, respectively. Using the marker Wx-B1, 204 lines (74.7%) were presumed to have the Wx-B1a allele and 69 (25.3%) possessed Wx-B1b. The over-expressing allele of Bx7 OE and subunit By8*, not clearly seen with SDS-PAGE, were detected by RP-HPLC. The numbers of lines with subunits Ax2*, By8, By9, Bx17, Bx20, Dx5, and Glu-B3j were 90, 16, 57, 5, 46, 118, and 33, respectively, in the 142 lines analyzed with molecular markers, and were consistent with the results obtained by SDS-PAGE, except for one line with the 1A.1R translocation. Subunits Ax1 and Ax2* at the Glu-A1 locus showed significantly better effects on all quality parameters than subunit Null. Subunits 5 + 10 gave significantly better effects for all parameters. Subunit Glu-A3b showed more positive effects than its alternative alleles on SDS-SV and SDS-sedimentation volume/protein content index (SPI). The allele Glu-B3g showed the best effect on SDS-SV and Alveograph W, whereas Glu-B3j, associated with the 1B.1R translocation, exhibited a strongly negative effect on all quality parameters.
536			_aGlobal Wheat Program
546			_aText in English
591			_aSpringer
594			_aINT2411|INT3297|INT2411|INT0368|INT0610
650		7	_aWheat _2AGROVOC _91310
650		7	_aQuality _2AGROVOC _91231
650		7	_aGlutenins _2AGROVOC _91137
650		7	_aGel electrophoresis _2AGROVOC _922497
650		7	_aHPLC _2AGROVOC _96176
650		7	_aGenetic markers _2AGROVOC _91848
700	1		_aTang Jian-Wei _916894
700	1		_aPeña-Bautista, R.J. _8INT0368 _gGlobal Wheat Program _9645
700	1		_aSingh, R.P. _gGlobal Wheat Program _8INT0610 _9825
700	1		_9913 _aXinyao He _gGlobal Wheat Program _8INT3297
700	0		_920832 _aXiaoyong Shen
700	0		_919599 _aYao Da-Nian
700	0		_9377 _aXianchun Xia
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
773	0		_tEuphytica _gv. 172, no. 2, p. 235-250 _dDordrecht (Netherlands) : Springer, 2010. _wG444298 _x0014-2336
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/795
942			_cJA _2ddc _n0
999			_c27830 _d27830