000			02135nab a22004097a 4500
001			G91200
003			MX-TxCIM
005			20220602212620.0
008			121211b |||p||p||||||| |z||| |
022			_a0133-3720
022			_a1788-9170 (Online)
040			_aMX-TxCIM
041			_aeng
090			_aCIS-5429
100	0		_aJiro Murakami _920394
245	1	0	_aEvaluation of a real-time quantitative PCR assay for an effective screening of genotypes with FHB resistance and low mycotoxin accumulation in wheat and barley
260			_c2008. _aSzeged (Hungary) : _bCereal Research Non-Profit Company,
340			_aComputer File|Printed
500			_aPeer-review
520			_aTo estimate fungal biomass in grains infected with Fusarium graminearum causing Fusarium Head Blight (FHB), a real-time PCR assay was applied for the quantification of the pathogen DNA based on the copy number of trichothecene synthesis gene (Tri5) sequence. Using 64 lines of wheat and barley in a field, we found significant correlations among the disease severity, deoxynivalenot (DON) accumulation and the pathogen DNA amount in the grains from each inoculated spike. The strong relationships were maintained in the susceptible population showing high DON accumulation but not the resistance population with low DON accumulation. These results indicate that the PCR assay could be useful discarding the germplasm very susceptible to FHB disease.
536			_aResearch and Partnership Program
546			_aTex in English
594			_aINT1237
595			_aCSC
650		7	_2AGROVOC _92705 _aFusarium
650		7	_2AGROVOC _910042 _aVomitoxin
650		7	_2AGROVOC _92331 _aGibberella zeae
650		7	_2AGROVOC _912563 _aPCR
700	1		_aLewis, J., _92354
700	1		_aEgusa, M. _927687
700	1		_aGargouri, S. _917738
700	0		_aMotoichiro Kodama _917001
700	1		_9826 _aDuveiller, E. _gDG's Office _8INT1237
700	0		_aBan Tomohiro _920784
773	0		_tCereal Research Communications _gv. 36, no. Suppl. B., p. 147-148 _dSzeged (Hungary) : Cereal Research Non-Profit Company, 2008. _wG445006 _x0133-3720
942			_cJA _2ddc
999			_c27289 _d27289