000 03025nab a22004217a 4500
001 G91151
003 MX-TxCIM
005 20220523213237.0
008 220523s2008 xxu|||p|op||| 00| 0 eng d
022 _a0031-949X
022 _a1943-7684 (Online)
024 8 _ahttps://doi.org/10.1094/PHYTO-98-12-1291
040 _aMX-TxCIM
041 _aeng
090 _aCIS-5402
100 0 _aDi Liu
_923532
245 1 2 _aA novel homeobox-like gene associated with reaction to stripe rust and powdery mildew in common wheat
260 _aSt. Paul, MN (USA) :
_bAmerican Phytopathological Society,
_c2008.
340 _aPrinted|Computer File
500 _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0031-949X
500 _aPeer review
500 _aOpen Access
520 _aStripe rust and powdery mildew, caused by Puccinia striiformis f. sp. tritici and Blumeria graminis f. sp. tritici, respectively, are severe diseases in wheat (Triticum aestivum) worldwide. In our study, differential amplification of a 201-bp cDNA fragment was obtained in a cDNA-amplified fragment length polymorphism (AFLP) analysis between near-isogenic lines Yr10NIL and Avocet S, inoculated with P. striiformis f. sp. tritici race CYR29. A full-length cDNA (1,357 bp) of a homeobox-like gene, TaHLRG (GenBank accession no. EU385606), was obtained in common wheat based on the sequence of GenBank accession AW448633 with high similarity to the above fragment. The genomic DNA sequence (2,396 bp) of TaHLRG contains three exons and two introns. TaHLRG appeared to be a novel homeobox-like gene, encoding a protein with a predicted 66-amino-acid homeobox domain. It was involved in race-specific responses to stripe rust in real-time quantitative polymerase chain reaction (PCR) analyses with Yr9NIL, Yr10NIL, and Avocet S. It was also associated with adult-plant resistance to stripe rust and powdery mildew based on the field trials of doubled haploid lines derived from the cross Bainong 64/Jingshuang 16 and two F2:3 populations from the crosses Lumai 21/Jingshuang 16 and Strampelli/Huixianhong. A functional marker, THR1 was developed based on the sequence of TaHLRG and located on chromosome 6A using a set of Chinese Spring nulli-tetrasomic lines.
536 _aGlobal Wheat Program
546 _aText in English
594 _aINT2411
650 7 _aWheat
_2AGROVOC
_91310
650 7 _aRusts
_2AGROVOC
_91251
650 7 _aPowdery mildews
_2AGROVOC
_95953
650 7 _aAmplified fragment length polymorphism
_2AGROVOC
_913624
650 7 _aDisease resistance
_2AGROVOC
_91077
700 0 _aXianchun Xia
_9377
700 1 _aHe Zhonghu
_gGlobal Wheat Program
_8INT2411
_9838
700 0 _aShichang Xu
_927592
773 0 _tPhytopathology
_n635399
_gv. 98, no. 12, p. 1291-1296
_dSt. Paul, MN (USA) : American Phytopathological Society, 2008.
_wG444676
_x0031-949X
856 4 _yOpen Access through DSpace
_uhttp://hdl.handle.net/10883/2680
942 _cJA
_2ddc
_n0
999 _c27268
_d27268