000			04321nab a22004457a 4500
001			G91148
003			MX-TxCIM
005			20220523215454.0
008			220523s2008 xxk|||p|op||| 00| 0 eng d
022			_a1601-5223 (Online)
024	8		_ahttps://doi.org/10.1111/j.1601-5223.2008.02046.x
040			_aMX-TxCIM
041			_aeng
090			_aCIS-5401
100	0		_aZhao Xian-lin _920761
245	1	0	_aCharacterization of novel LMW-GS genes at Glu-D3 locus on chromosome 1D in Aegilops tauschii
260			_aUnited Kingdom : _bWiley, _c2008.
340			_aComputer File|Printed
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0018-0661
500			_aPeer review
500			_aOpen Access
520			_aThe objectives of this study were to clarify the relationship between LMW-GS Glu-D3 gene of Ae. tauschii registered in GenBank and the six Glu-D3 genes including 12 allelic variants of common wheat characterized in our previous studies, and identify novel Glu-D3 genes and haplotypes from Ae. tauschii using gene specific PCR amplification. By searching the NCBI database, 13 LMW-GS genes/pseudogenes of Ae. tauschii were retrieved and classified into five gene families based on their nucleotide similarity with the six Glu-D3 genes of common wheat. Of them, four Ae. tauschii genes, AY585350, AY585354, AY585355 and AY585356 matched GluD3-4, GluD3-5, GluD3-1 and GluD3-2 of common wheat, respectively, and one pseudogene AY585351 matched to GluD3-6, but none of them matched to GluD3-3. In order to identify the Glu-D3 genes from Ae. tauschii corresponding to GluD3-3 and GluD3-6 of common wheat, gene specific primers were developed to amplify 8–18 Ae. tauschii entries. As a result, two novel Glu-D3 genes, designated as GluDt3-3 and GluDt3-6, were identified. GluDt3-3 showed seven allelic variants or haplotypes at the DNA level in eight Ae. tauschii entries, designated as GluDt3-31, GluDt3-32, GluDt3-33, GluDt3-34, GluDt3-35, GluDt3-36 and GluDt3-37, respectively. Two to eight SNPs were found among the seven haplotypes and 1–4 amino acid substitutions among the deduced peptides. Multiple sequence alignments showed that the DNA similarity was 99.6–99.9% among the seven GluDt3-3 haplotypes, and 99.4–99.7% between these haplotypes and those of common wheat GluD3-3 gene. GluDt3-6 presented seven haplotypes in 18 Ae. tauschii entries, designated as GluDt3-61, GluDt3-62, GluDt3-63, GluDt3-64, GluDt3-65, GluDt3-66 and GluDt3-67, respectively. GluDt3-61 from Ae. tauschii entry Ae38 was the only one haplotype with complete coding sequence, and the other six were all pseudogenes. Compared with GluD3-6 gene of common wheat, GluDt3-61 exhibited a 3-bp insertion, a 42-bp deletion and 11 base substitutions, leading to a glutamine insertion in position 52, 14 amino acid deletion in position 84–97 and 10 amino acid mutations in its deduced peptide; GluDt3-62 and GluDt3-63 showed a 6-bp insertion, a 24-bp deletion and 15–21 base substitutions in coding region, of which a nonsense mutation from C to T at position 622 resulted in pseudogenes; GluDt3-64 had five base substitution, including a nonsense mutation at the position 742. GluDt3-65, GluDt3-66 and GluDt3-67 all had a base deletion at position 247, as well as 7–8 base substitutions, which resulted in frameshift mutations in the three haplotypes. The results indicated that Ae. tauschii also contains six Glu-D3 genes and their allelic variants are even richer than those in common wheat.
536			_aGlobal Wheat Program
546			_aText in English
594			_aINT2411
650		7	_91000 _aAegilops _2AGROVOC
650		7	_92185 _aAlleles _2AGROVOC
650		7	_91848 _aGenetic markers _2AGROVOC
650		7	_91937 _aNucleotide sequence _2AGROVOC
650		7	_93635 _aMutation _2AGROVOC
700	0		_aYan Yang _98657
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
700	0		_aZhensheng Lei _917779
700	0		_aWujun Ma _91979
700	0		_aQixin Sun _922892
700	0		_aXianchun Xia _9377
773	0		_tHereditas _n635362 _gv. 145, no. 5, p. 238-250 _dUnited Kingdom : Wiley, 2008. _wG444334 _x1601-5223
856	4		_yOpen Access through DSpace _uhttp://hdl.handle.net/10883/2679
942			_cJA _2ddc _n0
999			_c27267 _d27267