000			02821nab a22004217a 4500
001			G91144
003			MX-TxCIM
005			20240826225837.0
008			210702s2008 ne |||p|op||| 00| 0 eng d
022			_a1572-9788 (Online)
022			_a1380-3743
024	8		_ahttps://doi.org/10.1007/s11032-008-9194-2
040			_aMX-TxCIM
041			_aeng
090			_aCIS-5399
100	1		_aWen, W.E. _920821
245	1	0	_aDevelopment of an STS marker tightly linked to Yr26 against wheat stripe rust using the resistance gene-analog polymorphism (RGAP) technique
260			_aDordrecht (Netherlands) : _bSpringer, _c2008.
340			_aComputer File|Printed
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1380-3743
520			_aThe gene Yr26 confers resistance to all races of Puccinia striiformis f. sp. tritici (PST), the casual pathogen of wheat stripe rust in China. Here, we report development of a molecular marker closely linked to Yr26 using a resistance gene-analog polymorphism (RGAP) technique. A total of 787 F2 plants and 165 F3 lines derived from the cross Chuanmai 42/Taichung 29 were used for linkage analysis. Eighteen near-isogenic lines (NILs) and 18 Chinese wheat cultivars and advanced lines with different genes for stripe rust resistance were employed for the validation of STS markers. A total of 1,711 RGAP primer combinations were used to test the parents and resistant and susceptible bulks. Five polymorphic RGAP markers were used for genotyping all F2 plants. Linkage analysis showed that the five RGAP markers were closely linked to Yr26 with genetic distances ranging from 0.5 to 2.9 cM. These markers were then converted into STS markers, one, CYS-5, of which was located 0.5 cM to Yr26 and was closely associated with the resistance gene when validated over 18 NILs and 18 Chinese wheat cultivars and lines. The results indicated that CYS-5 can be used in marker-assisted selection targeted at pyramiding Yr26 and other genes for stripe rust resistance.
536			_aGlobal Wheat Program
546			_aText in English
594			_aINT2411
650	1	7	_aMarker-assisted selection _2AGROVOC _910737
650	1	0	_aPolymorphism _gAGROVOC _93752
650	1	7	_aRusts _2AGROVOC _91251
650	1	7	_aTriticum aestivum _2AGROVOC _91296
700	1		_aLi, G.Q. _920751
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
700	1		_920755 _aYang, W.Y.
700	0		_918246 _aMingliang Xu
700	0		_9377 _aXianchun Xia
773	0		_tMolecular Breeding _n635360 _gv. 22, no. 4, p. 507-515 _dDordrecht (Netherlands) : Springer, 2008. _wG78961 _x1380-3743
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/622
942			_cJA _2ddc _n0
999			_c27265 _d27265