000 02421nab a22003617a 4500
001 G91026
003 MX-TxCIM
005 20190715202136.0
008 121211b |||p||p||||||| |z||| |
040 _aMX-TxCIM
100 1 _9875
_aBABU, R.
_gGlobal Maize Program
_8INT2925
245 0 0 _aHVA1, a LEA gene from barley confers dehydration tolerance in transgenic rice (Oryza sativa L.) via cell membrane protection
260 _c2004
340 _aComputer File
520 _aDrought is by far the leading environmental stress-limiting crop yields world-wide. Genetic engineering techniques hold great promise for developing crop cultivars with drought tolerance. Transgenic rice plants have been developed by engineering a wide variety of genes and were shown to be drought tolerant. Understanding the mechanism of stress tolerance in these transgenic plants under agronomically realistic stress conditions would further hasten breeding for drought resistance in rice. In this study, transgenic rice lines expressing the barley HVA1 gene were tested under prolonged drought stress cycle to understand the mechanism of dehydration tolerance. Transgenic plants maintained higher leaf relative water content (RWC) and showed lesser reduction in plant growth under drought stress as compared to non-transgenic (NT) plants. Maintenance of higher plant water status delayed wilting by more than 2 weeks in transgenic plants as compared to NT plants. Transgenic lines had relatively better cell membrane protection than NT line 28 days after stress. Both transgenic and NT lines had similar but low levels of osmotic adjustment (OA) under drought stress. The results indicated that the production of HVA1 proteins might have helped in better performance of transgenic rice plants by protecting cell membrane from injury under drought stress.
536 _aGlobal Maize Program
546 _aEnglish
591 _aNo CIMMYT affiliation|Elsevier
594 _aINT2925
650 1 0 _aCell membrane protection
650 1 0 _aDehydration tolerance
650 1 0 _aHVA1 gene
650 1 0 _aLEA protein
650 1 0 _aOsmotic adjustment
650 1 0 _aTransgenic rice
700 1 _aBlum, A.,
_ecoaut.
_9438
700 1 _aHo, T. H. D.,
_ecoaut.
700 1 _aNguyen, H.T.,
_ecoaut.
700 1 _aWu, R.,
_ecoaut.
700 1 _aZhang, J.,
_ecoaut.
773 0 _tPlant Science
_gv. 166, no. 4, p. 855-862
942 _cJA
_2ddc
999 _c27211
_d27211