000			03199nab a22003977a 4500
001			G90563
003			MX-TxCIM
005			20230904152542.0
008			210928s2008 cc |||p|op||| 00| 0 chi d
022			_a0578-1752
040			_aMX-TxCIM
041			_achi
090			_aCIS-5354
100	0		_aYingxiu Wan _95893
245	1	0	_aDevelopment of multiplex PCR and identification of major quality genes in cultivars from Yellow and Huai River Valley wheat region
260			_aBeijing (China) : _bAcademy of Agricultural Sciences, _c2008.
340			_aComputer File|Printed
500			_aPeer review
500			_aAbstract in English and Chinese
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0578-1752
520			_a(Objective) Wheat quality is controlled by many loci and each of them has several alleles. Development of multiplex PCR is very important to enhance the efficiency and reduce the cost in molecular marker assisted breeding for wheat quality traits. (Method) In the present study, the molecular markers for important quality trait genes, i.e., high-molecular-weight glutenin subunit (HMW-GS) genes Ax2*, Bx14, Bx17 and Dx5, and low-molecular-weight glutenin subunit (LMW-GS) gene Glu-A3d, as well as the marker BDFL-BRD for Wx-B1, MAG269 for Wx-D1, ƒÖ-sec for 1BL/1RS translocation and PPO18 for polyphenol oxidase(PPO) activity, were chosen. Three combinations of multiplex PCRs, PCR-I (Ax2*/Bx17/Dx5), PCR-II (Bx14/Glu-A3d/ƒÖ-sec) and PCR- III (BDFL-BRD/MAG269/PPO18) were built up according to genes controlling for pan bread and Chinese noodle quality, and the annealing temperatures of different markers. (Result) A total of 141 wheat cultivars from Yellow and Huai River Valley wheat region were evaluated adopting the multiplex PCRs, and 80 of them were previously assessed using SDS-PAGE for HMW-GS, LMW-GS and secalin, and the corresponding results were identical. Of the 141 wheat cultivars, the frequencies of Ax2*, Bx14 and Bx17 were 4.3%, 7.1%, and 1.4%, while Dx5 and Glu-A3d accounted for 17.7% and 27.7%, respectively. Presences of 1BL/1RS translocation and low polyphenol oxidase (PPO) activity genes were much higher, up to 44.0% and 51.8%, respectively. Wx-B1 null type accounted for 4.3%, and no Wx-D1 null type presented. (Conclusion) The results indicated that the three multiplex PCRs developed can be used to detect the nine genes stably and efficiently.
536			_aGlobal Wheat Program
546			_aText in Chinese
594			_aINT2411
650		7	_aWheat _2AGROVOC _91310
650		7	_aTriticum aestivum _2AGROVOC _91296
650		7	_aProcessing quality _2AGROVOC _99916
650		7	_aPCR _2AGROVOC _912563
700	0		_916893 _aZhang Xiao-Ke
700	0		_9377 _aXianchun Xia
700	0		_95892 _aPingzhi Zhang
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
773	0		_tScientia Agricultura Sinica _n635297 _gv. 41, no. 3, p. 643-653 _dBeijing (China) : Academy of Agricultural Sciences, 2008. _wG445218 _x0578-1752
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/1527
942			_cJA _2ddc _n0
999			_c27076 _d27076