000 04957nab a22004577a 4500
001 G90246
003 MX-TxCIM
005 20220518174749.0
008 220518s2007 cc |||p|op||| 00| 0 chi d
022 _a1875-2780 (Online)
022 _a0496-3490
040 _aMX-TxCIM
041 _achi
090 _aCIS-5163
100 0 _aHao Li
_918205
245 1 0 _aIsolation of differentially expressed genes from wheat cultivars Jinan 17 and Yumai 34 with good bread quality under heat stress during grain filling stage
260 _aBeijing, (China) :
_bInstitute of Crop Sciences,
_c2007.
340 _aComputer File|Printed
500 _aAbstract in Chinese and English.
500 _aPeer-review: No - Open Access: Yes|http://211.155.251.148:8080/zwxb/EN/column/column81.shtml
500 _aPeer review
500 _aOpen Access
520 _aStudies of gene expression patterns under heat stress during grain filling stage will provide important information for breeding wheat cultivars with high quality. In the present study, two wheat cultivars, Jinan 17 and Yumai 34 with different quality stability under various environments, were used to in the influence of high temperature on gene expression. The wheat plants were exposed to high temperature (38℃/25℃ day/night) for three days in the middle (from 15 to 18 days post-anthesis) and late stage (from 30 to 33 days post-anthesis) of grain filling in a climate chamber. Spikelets in middle of heads were harvested, and RNA of kernels was extracted with a combined technique of cold phenolic and Trizol single-step methods. cDNA was obtained by the reverse transcription of total RNA, and differential bands were detected subsequently in cDNA-AFLP analysis. In total, 410 and 316 differential bands were detected from Jinan 17 and Yumai 34, respectively. The differential fragments were cloned, sequenced and blasted in NCBI, and 85 and 99 positive fragments of differentially expressed genes under heat stress were obtained from Jinan 17 and Yumai 34, respectively. After the positive fragments were validated by reverse Northern blotting, 25 positive fragments isolated from Jinan 17 showed intense signal, and 22 of them were induced under heat stress, which were notablely homologous to stress response genes and heat shock protein of wheat. Meanwhile, 31 positive fragments showed intense signal were observed from Yumai 34, and 25 of them were suppressed, which were notablely homologous to stress response genes, ethylene forming enzyme, pyrroline-5-carboxylate synthetase. The rusults indicated that gene expression was more induced under heat stress in Jinan17, whereas suppressed more in Yumai 34, which might lead to differences in heat tolerance and quality stability. Five fragments from Jinan 17, and two fragments from Yumai 34 did not have any homology sequences in the BLAST analysis, while other fragments have homology protein or nucleic acid sequences in wheat or other crops. Two fragments induced in response to heat stress were notablely homologous to the storage protein genes, which might induce the expression of transcripts related to storage protein under heat stress. Fifteen differential fragments were detected from medium stage of grain filling in Jinan 17, whereas those from late stage were 10, while 29 and 2 differential fragments in Yumai 34 were observed in medium and late stages, respectively. This indicated that gene expression was more significantly affected by heat stress in medium stage than in late stage of grain filling, especially in Yumai 34. The difference of gene expression patterns between two wheat cultivars were observed, stress response genes were induced in Jinan 17, ethylene forming enzyme and pyrroline-5-carboxylate synthetase as well as stress response genes were suppressed in Yumai 34, which may result in the different responses in heat tolerance and quality stability. The identification and characterization of heat stress responsive genes in wheat may provide a molecular biological understanding of gene expression patterns and regulation involved in the heat stress in wheat.
536 _aGlobal Wheat Program
546 _aText in Chinese
594 _aINT2411
650 7 _aWheat
_2AGROVOC
_91310
650 7 _aSeed filling
_91911
_2AGROVOC
650 7 _aHeat stress
_91971
_2AGROVOC
650 7 _aAmplified fragment length polymorphism
_913624
_2AGROVOC
650 7 _98835
_aGene Expression
_2AGROVOC
650 7 _aQuality
_2AGROVOC
_91231
650 7 _aStability
_96345
_2AGROVOC
700 0 _aZhang Ping-ping
_923183
700 0 _aZha Xiang-Dong
_927515
700 0 _aXianchun Xia
_9377
700 1 _aHe Zhonghu
_gGlobal Wheat Program
_8INT2411
_9838
773 0 _tActa Agronomica Sinica
_n635011
_gv. 33, no. 10, p. 1644-1653
_dBeijing, (China) : Institute of Crop Sciences, 2007.
_wG446116
_x0496-3490
856 4 _yOpen Access through DSpace
_uhttp://hdl.handle.net/10883/2612
942 _cJA
_2ddc
_n0
999 _c26924
_d26924