000			02584nab a22003737a 4500
001			G89947
003			MX-TxCIM
005			20230914204317.0
008			210920s2007 cc |||p|op||| 00| 0 chi d
022			_a0578-1752
040			_aMX-TxCIM
041			_achi
090			_aCIS-5020
100	0		_aZhang Ping-ping _923183
245	1	0	_aProtocol establishment of reversed-phase high-performance liquid chromatography (RP-HPLC) for analyzing wheat gluten protein
260			_aBeijing (China) : _bAcademy of Agricultural Sciences, _c2007.
340			_aComputer File|Printed
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0578-1752
520			_a(Objective) This study will establish a protocol of reversed-phase high-performance liquid chromatography (RP-HPLC) used in separating and quantifying wheat gluten protein in China, which has been widely used in wheat quality study since the 1980s. (Method) Two cultivars, Zhongyou 9507 (strong gluten) and Jing 411 (weak gluten), were chosen to study the effect of temperature, elution gradient, sample weight, extraction duration, and injection volume on gluten protein resolution. The ability to repeat experimental results was validated. (Result) The results indicated that the gliadin and glutenin subunits extraction rate reached 90% when 45mg of flour was extracted with 1 ml 50% aqueous 1-propanol (v/v) and glutenin extraction buffer (50% 1-propanal (v/v) +2 mol L-1 Urea+0.2 mol L-1 Tris-HCl (pH 6.6)+1%DTT), respectively. The optimal sample injection volume of gliadin and glutenin was 10-15 and 15-20, respectively. The modified elution gradient effectively separated gliadin and glutenin subunits. (Conclusion) This protocol can qualify and quantify the components of gliadin and glutenin accurately combining SDS-PAGE, by which we can predicate the wheat end-use quality.
536			_aGlobal Wheat Program
546			_aText in Chinese
594			_aINT2411
650		7	_aWheat _2AGROVOC _91310
650		7	_aGluten _2AGROVOC _91974
650		7	_aProteins _2AGROVOC _91224
650		7	_aHPLC _2AGROVOC _96176
700	0		_aYong Zhang _91857
700	0		_aXianchun Xia _9377
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
773	0		_tScientia Agricultura Sinica _n634771 _gv. 40, no. 5, p. 1002-1009 _dBeijing (China) : Academy of Agricultural Sciences, 2007. _wG445218 _x0578-1752
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/1428
942			_cJA _2ddc _n0
999			_c26744 _d26744