000			02078nab a22003737a 4500
001			G89702
003			MX-TxCIM
005			20220511204025.0
008			220511s2006 nr |||p|op||| 00| 0 eng d
022			_a1684-5315
024			_2https://doi.org/10.5897/AJB06.431 _qDOI is wrong in original article
040			_aMX-TxCIM
041			_aeng
090			_aCIS-4930
100	1		_aDanson, J. _97404
245	1	0	_aDetection of Bacillus thuringiensis genes in transgenic maize by the PCR method and FTA paper technology
260			_aNigeria : _bAcademic Journals, _c2006.
340			_aComputer File|Printed
500			_aPeer-review: No - Open Access: Yes|http://www.ajol.info/index.php/ajb
500			_aPeer review
500			_aOpen Access
520			_aWe optimized the PCR method to detect genetically engineered Bacillus thuringiensis (Bt) maize in open quarantine fields in Kenya. Many factors affect the extraction of the DNA from plants, such as the amount of tissue available, the condition of the plant material, the numbers of steps involved in the extraction procedure, and the required purity needed. We tested the application of the FTA paper technology for field sampling whereby leaf materials are not removed and transported from the site. We also applied the PCR method, a technique that is widely used for detection of genetically modified organism (GMOs). We used primers specific to the Bt genes present in the transgenic maize to screen for the Bt genes Cry 1Ab and Cry 1Ba.
546			_aText in English
591			_a Academic Journals
650		7	_aGenetically modified organisms _2AGROVOC _910536
650		7	_aMaize _2AGROVOC _91173
650		7	_aBacillus thuringiensis _2AGROVOC _96672
650		7	_aPCR _2AGROVOC _912563
700	1		_aKimani, M., _ecoaut.
700	1		_aMbogori, M., _ecoaut.
773	0		_tAfrican Journal of Biotechnology _n634572 _gv. 5, no. 22, p. 2345-2349 _dNigeria : Academic Journals, 2006. _wG76647 _x1684-5315
856	4		_yOpen Access through DSpace _uhttp://hdl.handle.net/10883/3034
942			_cJA _2ddc _n0
999			_c26578 _d26578