000			04043nab a22004577a 4500
001			G89516
003			MX-TxCIM
005			20220607160106.0
008			210701s2006 gw |||p|op||| 00| 0 eng d
022			_a1432-2242 (Online)
022			_a0040-5752
024	8		_ahttps://doi.org/10.1007/s00122-006-0379-y
040			_aMX-TxCIM
041			_aeng
090			_aCIS-4832
100	0		_aZhao Xian-lin _920761
245	1	0	_aCharacterization of three low-molecular-weight Glu-D3 subunit genes in common wheat
260			_aBerlin (Germany) : _bSpringer, _c2006.
340			_aComputer File|Printed
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
520			_aLow-molecular-weight glutenins (LMW-GS) in common wheat (Triticum aestivum L.) are of great importance for processing quality of pan bread and noodles. The objectives of this study are to identify LMW-GS coding genes at GluD3 locus on chromosome 1D and to establish relationships between these genes and GluD3 alleles (a, b, c, d, and e) defined by protein electrophoretic mobility. Specific primer sets were designed to amplify each of the three LMW-GS chromosome 1D gene regions including upstream, coding and downstream regions of eight wheat cultivars containing GluD3 a, b, c, d and e alleles. Three LMW-GS genes, designated as GluD3-1, GluD3-2 and GluD3-3, were amplified from the eight wheat cultivars. The allelic variants of these three genes were analysed at the DNA and protein level. GluD3-1 showed two allelic variants or haplotypes, one common to cultivars containing protein alleles a, d and e (designated GluD3-11) and the other was present in cultivars with alleles b and c (designated GluD3-12). Comparing with GluD3-12, a 3-bp deletion was found in the coding region of the N-terminal repetitive domain of GluD3-11, leading to a glutamine deletion at the 116th position. GluD3-2 had three variants at the DNA level in the eight cultivars, which were designated as GluD3-21, GluD3-22 and GluD3-23. In comparison to GluD3-21, a single nucleotide polymorphism (SNP) was detected for GluD3-22 in the signal peptide region, resulting in an amino acid change from alanine to threonine at the 11th position; and 11 mutations were found at GluD3-23, with five in upstream region, four in coding region and two in downstream region, respectively. GluD3-3 had two haplotypes, designated as GluD3-31 and GluD3-32, both belonging to LMW-s glutenin subunits though their first amino acids in N-terminal region are different. Compared with the GenBank GluD3 genes, nucleotide sequences of GluD3-21 and GluD3-23 were the same as X13306 and AB062875, respectively. GluD3-22 and GluD3-11 had only one-base difference from U86027 and AB062865. GluD3-12 was not found in the GenBank database, indicating a newly identified GluD3 gene variation. GluD3-3 was a new gene different from any other known GluD3 genes. Analyses of the relationship between Glu-D3 alleles defined by protein electrophoretic mobility and different GluD3 gene variations at the DNA or protein level provided molecular basis for DNA based identification of glutenin alleles.
536			_aGlobal Wheat Program
546			_aText in English
591			_aSpringer
594			_aINT2411
650		7	_2AGROVOC _91310 _aWheat
650		7	_2AGROVOC _91137 _aGlutenins
650		7	_2AGROVOC _99916 _aProcessing quality
650		7	_2AGROVOC _93563 _aGenes
650		7	_2AGROVOC _92084 _aChromosome mapping
700	0		_aXianchun Xia _9377
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
700	1		_913592 _aGale, K.
700	0		_917779 _aZhensheng Lei
700	1		_91980 _aAppels, R.
700	0		_91979 _aWujun Ma
773	0		_tTheoretical and Applied Genetics _n634435 _gv. 113, no. 7, p. 1247-1259 _dBerlin (Germany) : Springer, 2006. _wG444762 _x0040-5752
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/650
942			_cJA _2ddc _n0
999			_c26472 _d26472