000 03099nab a22004217a 4500
001 G89269
003 MX-TxCIM
005 20230918165804.0
008 210909s2006 xxk|||p|op||| 00| 0 eng d
022 _a1365-3059
024 8 _ahttps://doi.org/10.1111/j.1365-3059.2006.01391.x
040 _aMX-TxCIM
041 _aeng
090 _aCIS-4779
100 1 _aMercado Vergnes, D.
_922893
245 1 0 _aIdentification of Alternaria spp on wheat by pathogenicity assays and sequencing
260 _aUnited Kingdom :
_bWiley,
_c2006.
340 _aComputer File|Printed
500 _aPeer review
500 _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0032-0862
520 _aThe pathogenicity of Alternaria spp. isolated from wheat leaves collected in regions where alternaria leaf blight has been reported was compared with that of IMI reference isolates of A. triticina and A. alternata using two durum and two bread wheat genotypes. To identify isolates putatively corresponding to A. triticina, morphological and DNA sequence analyses based on ribosomal DNA from the internal transcribed spacer (ITS) region (ITS1, 5·8S rRNA gene, ITS2) and toxicity bioassays of culture filtrate were combined. Glasshouse inoculations provided reliable information to assess the pathogenicity of A. triticina isolates on wheat. Alternaria leaf blight symptoms were produced by the A. triticina isolates only on durum wheat cv. Bansi, while A. alternata, A. tenuissima and A. arborescens isolates were found to be nonpathogenic on the wheat cultivars tested. Alternaria triticina isolates were distinguished from other Alternaria species by Simmons and Roberts’ sporulation pattern 6 and two to three conidia per sporulation unit associated with primary conidia bearing long (> 7 µm) apical secondary conidiophores. Phylogenetic analysis also proved effective at discriminating wheat-pathogenic A. triticina from other nonpathogenic Alternaria species. Alternaria triticina isolates yielded longer ITS sequences than A. alternata, A. tenuissima and A. arborescens isolates, leading to clear-cut differences as visualized with agarose gel electrophoresis. Additionally, only culture filtrates of A. triticina isolates caused nonspecific necrotic lesions on leaves of 3-week-old wheat plants.
536 _aResearch and Partnership Program
546 _aText in English
591 _a0706
594 _aINT1237
650 7 _2AGROVOC
_931630
_aAlternaria
650 7 _2AGROVOC
_95348
_aBlight
650 7 _2AGROVOC
_92377
_aMycotoxins
650 7 _2AGROVOC
_91296
_aTriticum aestivum
650 7 _2AGROVOC
_91297
_aTriticum durum
650 7 _2AGROVOC
_91134
_aGenotypes
700 1 _aRenard, M.E.
_922895
700 1 _9826
_aDuveiller, E.
_gDG's Office
_8INT1237
700 1 _922659
_aMaraite, H.
773 0 _tPlant Pathology
_n634205
_gv. 55, no. 4, p. 485-493
_dUnited Kingdom : Wiley, 2006.
_wG444692
_x1365-3059
856 4 _yAccess only for CIMMYT Staff
_uhttps://hdl.handle.net/20.500.12665/1366
942 _cJA
_2ddc
_n0
999 _c26302
_d26302