000			02851nab a22003977a 4500
001			G83058
003			MX-TxCIM
005			20230918232840.0
008			210701s2005 cc |||p|op||| 00| 0 chi d
022			_a0578-1752
040			_aMX-TxCIM
041			_achi
090			_aCIS-4685
100	0		_aXu Zhao-hua _920746
245	1	0	_aAnalysis of waxy proteins in Chinese winter wheat cultivars using SDS-PAGE and molecular markers
260			_aBeijing (China) : _bAcademy of Agricultural Sciences, _c2005.
340			_aPrinted|Computer File
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0578-1752
520			_aA total of 306 wheat cultivars and advanced lines from China, US and Australia were screened for the Waxy protein by SDS-PAGE. The results indicated that 46 of these cultivars were the null Wx-B1 type. The proportion of individuals with null Wx-B1 in 120 F2 progenies derived from the Jimai19×Yumai47 was 1/4 approximately, in accordance with the theoretical ratio. Three STS and 1 SSR markers were used to analyze the wheat cultivars with different types of Waxy proteins. Validation of the markers was carried out for the detection of Wx-7A, Wx-4A and Wx-7D genes. The results indicated that a 1172 bp-fragment was amplified with Wx-7A specific STS marker from the wild genotypes, while the fragment was absent for the mutant with null Wx-A1; a 440 bp-fragment was detected with Wx-4A specific STS marker from the wild genotypes, whereas, the fragment was absent for the mutant with null Wx-B1; a 940 bp-fragment was amplified with Wx-7D specific STS marker from the wild genotypes, and a 360 bp-fragment was found for the mutant such as ‘Baihuomai’ with null Wx-D1; and a 204 bp-fragment was detected with Wx-7D specific SSR marker from the wild genotypes, while the fragment was absent for the mutant with null Wx-D1. These markers are useful tools to identify wheat cultivars with mutant and normal alleles of the Waxy genes in marker-assisted selection of wheat breeding programme.
536			_aGlobal Wheat Program
546			_aText in Chinese
591			_a0603
594			_aINT2411
650		7	_2AGROVOC _91296 _aTriticum aestivum
650		7	_2AGROVOC _91224 _aProteins
650		7	_2AGROVOC _922497 _aGel electrophoresis
650		7	_2AGROVOC _91848 _aGenetic markers
700	0		_aLanqin Xia _920737
700	0		_aXinmin Chen _95907
700	0		_aXianchun Xia _9377
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
773	0		_tScientia Agricultura Sinica _n633973 _gv. 38, no. 8, p. 1514-1521 _dBeijing (China) : Academy of Agricultural Sciences, 2005. _wG445218 _x0578-1752
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/626
942			_cJA _2ddc _n0
999			_c26176 _d26176