| 000 | 02489nab a22003737a 4500 | ||
|---|---|---|---|
| 999 |
_c26136 _d26136 |
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| 001 | G82972 | ||
| 003 | MX-TxCIM | ||
| 005 | 20211006082400.0 | ||
| 008 | 210630s2007 cc |||p|op||| 00| 0 chi d | ||
| 022 | _a0578-1752 | ||
| 040 | _aMX-TxCIM | ||
| 041 | _achi | ||
| 090 | _aCIS-4624 | ||
| 100 | 0 |
_aGenying Li _95949 |
|
| 245 | 1 | 0 | _aConstruction of expression vector harboring Pina & Pinb fused gene and transformation into durum wheat |
| 260 |
_aBeijing (China) : _bAcademy of Agricultural Sciences, _c2007. |
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| 340 | _aComputer File|Printed | ||
| 500 | _aPeer review | ||
| 500 | _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0578-1752 | ||
| 520 | _a[Objective] Construction of expression vector harboring the fragment of fused Pina and Pinb, and verification of Pina and Pinb function. [Method] In the present study, a highly efficient expression vector (pFUPaPb) that harbors the fused fragment of Pina Pinb flanked with regulation sequences of and poly (A) was constructed, employing Ubiqutin as a promoter and bar gene as a selective marker. Common wheat variety Jing 411 is the donor of Pina and Pinb genes. pFUPaPb was transferred into durum wheat by biolistic method. [Result] After screening with bialophos and PCR identification with gene specific primers and dot blotting confirmation, 35 transplants were obtained. The expression of fused genes was detected in 2 of 16 transplants examined. The SKCS values of these two lines were decreased 10 and 12, respectively. Among the three medium used for callus inducing SD2 medium was the comparatively efficient medium for callus induction of durum immature embryo. MS+8 mgL-1 zeatin is good for callus regeneration. [Conclusion] Co-expression of Pina and Pinb decreased the endosperm texture of durum wheat. | ||
| 536 | _aGlobal Wheat Program | ||
| 546 | _aText in Chinese | ||
| 594 | _aINT2411 | ||
| 650 | 1 | 0 |
_aDurum wheat _91086 |
| 650 | 1 | 0 | _aImmature embryo culture |
| 650 | 1 | 0 | _aPina Pinb fused expression vector |
| 650 | 1 | 0 | _aTransformation |
| 700 | 0 |
_aLanqin Xia _920737 |
|
| 700 | 0 |
_aXianchun Xia _9377 |
|
| 700 | 1 |
_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838 |
|
| 773 | 0 |
_tScientia Agricultura Sinica _n633897 _gv. 40, no. 7, p. 1315-1323 _dBeijing (China) : Academy of Agricultural Sciences, 2007. _wG445218 _x0578-1752 |
|
| 856 | 4 |
_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/546 |
|
| 942 |
_cJA _2ddc _n0 |
||