000			03453nab a22004457a 4500
001			G82839
003			MX-TxCIM
005			20230223182849.0
008			210908s2006 gw |||p|op||| 00| 0 eng d
022			_a1432-2242 (Online)
022			_a0040-5752
024	8		_ahttps://doi.org/10.1007/s00122-005-0095-z
040			_aMX-TxCIM
041			_aeng
090			_aCIS-4601
100	1		_aChen, F. _920812
245	1	0	_aMolecular and biochemical characterization of puroindoline a and b alleles in Chinese landraces and historical cultivars
260			_aBerlin (Germany) : _bSpringer, _c2006.
340			_aPrinted|Computer File
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
520			_aKernel hardness that is conditioned by puroindoline genes has a profound effect on milling, baking and end-use quality of bread wheat. In this study, 219 landraces and 166 historical cultivars from China and 12 introduced wheats were investigated for their kernel hardness and puroindoline alleles, using molecular and biochemical markers. The results indicated that frequencies of soft, mixed and hard genotypes were 42.7, 24.3, and 33.0%, respectively, in Chinese landraces and 45.2, 13.9, and 40.9% in historical cultivars. The frequencies of PINA null, Pinb-D1b and Pinb-D1p genotypes were 43.8, 12.3, and 39.7%, respectively, in hard wheat of landraces, while 48.5, 36.8, and 14.7%, respectively, in historical hard wheats. A new Pinb-D1 allele, designated Pinb-D1t, was identified in two landraces, Guangtouxianmai and Hongmai from the Guizhou province, with the characterization of a glycine to arginine substitution at position 47 in the coding region of Pinb gene. Surprisingly, a new Pina-D1 allele, designated Pina-D1m, was detected in the landrace Hongheshang, from the Jiangsu province, with the characterization of a proline to serine substitution at position 35 in the coding region of Pina gene; it was the first novel mutation found in bread wheat, resulting in a hard endosperm with PINA expression. Among the PINA null genotypes, an allele designed as Pina-D1l, was detected in five landraces with a cytosine deletion at position 265 in Pina locus; while another novel Pina-D1 allele, designed as Pina-D1n, was identified in six landraces, with the characterization of an amino acid change from tryptophan-43 to a ‘stop’ codon in the coding region of Pina gene. The study of puroindoline polymorphism in Chinese wheat germplasm could provide useful information for the further understanding of the molecular basis of kernel hardness in bread wheat.
536			_aGlobal Wheat Program
546			_aText in English
591			_aSpringer|0601
594			_aINT2411
650		7	_91265 _aSoft wheat
650		7	_2AGROVOC _91296 _aTriticum aestivum
650		7	_2AGROVOC _91168 _aKernels
650		7	_2AGROVOC _92185 _aAlleles
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
700	0		_9377 _aXianchun Xia
700	1		_920407 _aXia L.Q.
700	1		_922878 _aZhang, X.Y.
700	1		_91659 _aLillemo, M.
700	1		_95838 _a Morris, C.F.
773	0		_tTheoretical and Applied Genetics _n633735 _gv. 112, no. 3, p. 400-409 _dBerlin (Germany) : Springer, 2006. _wG444762 _x0040-5752
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/1424
942			_cJA _2ddc _n0
999			_c26044 _d26044