| 000 | 03034nab a22005417a 4500 | ||
|---|---|---|---|
| 001 | G80394 | ||
| 003 | MX-TxCIM | ||
| 008 | 121211b |||p||p||||||| |z||| | | ||
| 022 | 0 | _a0026-8925 | |
| 040 | _aMX-TxCIM | ||
| 082 | 0 | 4 | _a95-063684 |
| 100 | 1 | _aUrao, T. | |
| 245 | 0 | 0 | _aTwo genes that encode Ca(2+)-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana |
| 260 | _c1994 | ||
| 340 | _aPrinted | ||
| 500 | _a4 ill., 2 graphs. Bibliography 56 ref. Summary (En) | ||
| 520 | _aTwo cDNA clones, cATCDPK1 and cATCDPK2, encoding Ca(2+)-dependent, calmodulinindependent protein kinases (CDPK) were cloned from Arabidopsis thaliana and their nucleotide sequences were determined. Northern blot analysis indicated that the mRNAs corresponding to the ATCDPK1 and ATDPK2 genes are rapidly induced by drought and high-salt stress but not by low-temperature stress or heat stress. Treatment of Arabidopsis plants with exogenous abscisic acid (ABA) had no effect on the induction of ATCDPK1 or ATCDPK2. These findings suggest that a change in the osmotic potential of the environment can serve as a trigger for the induction of ATCDPK1 and ATCDPK2. Putative proteins encoded by ATCDPK1 and ATCDPK2 which contain open reading frames of 1479 and 1488 bp, respectively, are designated ATCDPK1 and ATCDPK2 and show 52% identity at the amino acid sequence level. ATCDPK1 and ATCDPK2 exhibit significant similarity to a soybean CDPK (51% and 73%, respectively). Both proteins contain a catalytic domain that is typical of serine/threonine protein kinases and a regulatory domain that is homologous to the Ca(2+)-binding sites of calmodulin. Genomic Southern blot analysis suggests the existence of a few additional genes that are related to ATCDPK1 and ATCDPK2 in the Arabidopsis genome. The ATCDPK2 protein expressed in Escherichia coli was found to phosphorylate casein and myelin basic protein preferentially, relative to a histone substrate, and required Ca(2+) for activation | ||
| 546 | _aEnglish | ||
| 595 | _aAC | ||
| 650 | 1 | 0 | _aAcids |
| 650 | 1 | 0 | _aAlkaline earth metals |
| 650 | 1 | 0 | _aArabidopsis |
| 650 | 1 | 0 | _aCell structure |
| 650 | 1 | 0 | _aChemical resistance |
| 650 | 1 | 0 | _aChromosomes |
| 650 | 1 | 0 | _aCruciferae |
| 650 | 1 | 0 | _aElements |
| 650 | 1 | 0 | _aEnzymes |
| 650 | 1 | 7 |
_aGenomes _gAGROVOC _2 _91131 |
| 650 | 1 | 0 | _aMetallic elements |
| 650 | 1 | 0 | _aNucleic acids |
| 650 | 1 | 0 | _aNucleic compounds |
| 650 | 1 | 0 | _aNucleus |
| 650 | 1 | 0 | _aOrganic acids |
| 650 | 1 | 0 | _aPhosphotransferases |
| 650 | 1 | 7 |
_aPlant genetics and breeding _gNOT IN AGROVOC _2 _91208 |
| 650 | 1 | 0 | _aResistance to injurious factors |
| 650 | 1 | 0 | _aRNA |
| 650 | 1 | 0 |
_aStress _91277 |
| 650 | 1 | 0 | _aTransferases |
| 700 | 1 |
_aHayashida, N., _ecoaut. |
|
| 700 | 1 |
_aKatagiri, T., _ecoaut. |
|
| 700 | 1 |
_aMizoguchi, T., _ecoaut. |
|
| 700 | 1 |
_aShinozaki, K., _ecoaut. _9306 |
|
| 700 | 1 |
_aYamaguchi Shinozaki, K., _ecoaut. _9380 |
|
| 773 | 0 |
_tMolecular and General Genetics _gv. 244, no. 4, p. 331-340 |
|
| 942 | _cJA | ||
| 999 |
_c24910 _d24910 |
||