000			02800nab a22004097a 4500
001			G78548
003			MX-TxCIM
005			20230928185538.0
008			210630s2004 ne |||p|op||| 00| 0 eng d
022			_a0167-6857
022			_a1573-5044 (Online)
024	8		_ahttps://doi.org/10.1023/B:TICU.0000018389.99656.d8
040			_aMX-TxCIM
041			_aeng
072		0	_aF30
072		0	_aF63
090			_aCIS-3820
100	1		_aPellegrineschi, A. _96676
245	1	0	_aEffect of 2,4-Dichlorophenoxyacetic acid and NaCl on the establishment of callus and plant regeneration in durum and bread wheat
260			_aDordrecht (Netherlands) : _bSpringer, _c2004.
340			_aComputer File
500			_aPeer review
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0167-6857
520			_aOptimal callus induction and plant regeneration were obtained in bread and durum wheat by manipulating the NaCl concentration in the induction medium. Immature embryos from a high regeneration line of spring wheat (Triticum aestivum L.), 'MPB-Bobwhite 26', and an elite durum wheat (Triticum turgidum var. durum L.), 'Mexicali', were cultured in E3 induction medium consisting of Murashige and Skoog (MS) medium, 2.5 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D), 2% sucrose and 0.9% Bacto agar. The treated embryos were transferred to E3 liquid medium supplemented with various levels of 2,4-D and NaCl. Incubation on medium containing 2.5 mg l−1 2,4-D for 45 days produced callus and plant regeneration in 'MPB-Bobwhite 26', but lower callus yield and plant regeneration in 'Mexicali', indicating that 2,4-D alone was not sufficient for callus induction and plant regeneration in this durum variety. Callus yield and regeneration frequencies were higher in 'Mexicali' embryos that were incubated in media containing 2 mg l−1 2,4-D and 2 mg l−1 NaCl. The presence of NaCl in the medium beyond the initiation phase was detrimental to plant regeneration. The use of NaCl in the callus formation could form the basis for improved transformation of durum wheat varieties.
546			_aText in English
591			_a0209|Springer|AL-ABC Program|R01ABTR
650		7	_91310 _aWheat _2AGROVOC
650		7	_927634 _aEmbryonic development _2AGROVOC
650		7	_916962 _aSodium chloride _2AGROVOC
650		7	_931083 _aArtificial regeneration _2AGROVOC
700	1		_aBrito, R.M. _920732
700	1		_aMcLean, S. _95532
700	1		_aHoisington, D. _93643
773	0		_tPlant Cell, Tissue and Organ Culture _n629707 _gv. 77, no. 3, p. 245-250 _dDordrecht (Netherlands) : Springer, 2004. _wG78966 _x0167-6857
856	4		_yAccess only for CIMMYT Staff _uhttps://hdl.handle.net/20.500.12665/727
942			_cJA _2ddc _n0
999			_c24438 _d24438