000 01906nam a22004217a 4500
001 G54450
003 MX-TxCIM
008 121211s ||||f| 0 p|p||0|| |
020 _a968-6127-39
040 _aMX-TxCIM
041 0 _aEn
043 _aQY
072 0 _aH20
082 0 4 _a91-013768
100 1 _aForde, S.M.D.
111 2 _aWorld Perspectives on Barley Yellow Dwarf International Workshop
_cUdine (Italy)
_d6-11 Jul 1987
245 0 0 _aDifferentiation of barley yellow dwarf virus with monoclonal antibodies by immunosorbent electron microscopy
260 _c1990
340 _aPrinted
500 _a6 ref
520 _aThree strain-specific monoclonal antibodies (McAb), MAC 91, MAC 92, and MAFF2, were compared with two polyclonal antisera for the diagnosis of BYDV strains in immunosorbent electron microscopy. Used at an optimal dilution (1 ug/ml), the three McAbs specifically trapped the Rothamsted PAV-, RPV- and MAV-like isolates, unlike the polyclonal antisera, which showed considerable heterologous reaction. The test provides a simple and sensitive diagnostic method for BYDV, suitable for small immunosorbent assay results
546 _aEnglish
591 _aWorld Perspectives on Barley Yellow Dwarf International Workshop. Udine (Italy). 6-11 Jul 1987 CIMMYT, Ap. 6-641, 06600 Mexico, D.F. - Mexico|COMOD
595 _aAC
650 1 0 _aAntibodies
650 1 0 _aBiological development
650 1 0 _aImmunoenzyme techniques
650 1 0 _aImmunological factors
650 1 0 _aImmunological techniques
650 1 0 _aImmunology
650 1 0 _aLuteoviruses
650 1 7 _aPlant diseases
_gAGROVOC
_2
_91206
650 1 0 _aPlant viruses
_91543
650 1 0 _91306
_aViruses
_gAGROVOC
700 1 _aBarker, I.,
_ecoaut.
_91475
773 0 _tBurnett, P.A. (ed.). World perspectives on barley yellow dwarf. Mexico, DF (Mexico). CIMMYT. 1990. p. 194-196
_n91-013768
942 _cREP
999 _c2356
_d2356