000			02258nam a22004937a 4500
001			G54419
003			MX-TxCIM
008			121211s ||||f| 0 p|p||0|| |
020			_a968-6127-39
040			_aMX-TxCIM
041	0		_aEn
043			_aQY
072		0	_aH20
082	0	4	_a91-013910
100	1		_aNazare Pereira, A.M.
111	2		_aWorld Perspectives on Barley Yellow Dwarf International Workshop _cUdine (Italy) _d6-11 Jul 1987
245	0	0	_aStability of ELISA activity in frozen samples of barley yellow dwarf virus-infected leaf and leaf extracts
260			_c1990
340			_aPrinted
500			_a10 ref
520			_aWhen replicate samples of barley yellow dwarf virus-infected intact oat (Avena sativa L.) leaf, leaf extracts in phosphate buffer, or chloroform-clarified leaf extracts, were stored frozen at -20 deg C, -80 deg C, or in liquid nitrogen, their enzyme-linked immunosorbent assay (ELISA) activities fluctuated over time and according to the virus isolate involved. With the Purdue (P)-PAV isolate, no significant decrease of ELISA activity was noted with leaf, crude leaf extracts, or clarified extracts stored for up to 4 weeks at -20 deg C, -80 deg C, or in liquid nitrogen, except that clarified extracts stored at -20 deg C deteriorated after 1 week.
546			_aEnglish
591			_aWorld Perspectives on Barley Yellow Dwarf International Workshop. Udine (Italy). 6-11 Jul 1987 CIMMYT, Ap. 6-641, 06600 Mexico, D.F. - Mexico|COMOD
595			_aAC
650	1	0	_aAvena
650	1	0	_aChemicophysical properties
650	1	0	_aDevelopmental stages
650	1	0	_aFreezants
650	1	0	_aGramineae
650	1	0	_aImmunoenzyme techniques
650	1	0	_aImmunological techniques
650	1	0	_aInorganic acid salts
650	1	0	_aLuteoviruses
650	1	7	_aPlant anatomy _gAGROVOC _2 _91202
650	1	7	_aPlant diseases _gAGROVOC _2 _91206
650	1	0	_aPlant vegetative organs
650	1	0	_aPlant viruses _91543
650	1	0	_aProcessing aids
650	1	0	_aSalts
650	1	0	_91306 _aViruses _gAGROVOC
700	1		_aLister, R.M., _ecoaut.
773	0		_tBurnett, P.A. (ed.). World perspectives on barley yellow dwarf. Proceedings. Mexico, DF (Mexico). CIMMYT. 1990. p. 181-185 _n91-013910
942			_cREP
999			_c2325 _d2325