| 000 | 02539nab a22003977a 4500 | ||
|---|---|---|---|
| 001 | G80287 | ||
| 003 | MX-TxCIM | ||
| 005 | 20211006080528.0 | ||
| 008 | 121211b |||p||p||||||| |z||| | | ||
| 022 | 0 | _a0027-8424 | |
| 040 | _aMX-TxCIM | ||
| 082 | 0 | 4 | _a92-063003 |
| 100 | 1 | _aHershberger, R.J. | |
| 245 | 0 | 0 | _aMutator activity in maize correlates with the presence and expression of the Mu transposable element Mu9 |
| 260 | _c1991 | ||
| 340 | _aPrinted | ||
| 500 | _aill. references US (DNAL 500 N21P) | ||
| 520 | _aMutator is a powerful system for generating new mutants in maize. Mutator activity is attributable to a family of transposable, multicopy Mu elements, but none of the known elements is an autonomous (regulatory) element. This paper reports the discovery of Mu9, a 4942-base-pair Mu element that was cloned after it transposed into the Bronze-2 locus. Like other Mu elements, Mu9 has approximately 215-base-pair terminal inverted repeats and creates a 9-base-pair host sequence duplication upon insertion. A small gene family of elements that cross-hybridize to Mu9 has been found in all maize lines, and one of the other known Mu elements, Mu5, probably arose as a deletion of Mu9. Mu9 has several of the properties expected for the proposed regulator of Mutator activity. (i) The presence of Mu9 parallels the presence of Mutator activity in individuals from a line that genetically segregates for the Mu regulator. (ii) Lines that transmit Mutator to >90% of their progeny have multiple copies of Mu9. (iii) Most maize lines that lack Mutator activity and that are not descended from Mutator lines lack the Mu9 element. (iv) Transcripts that hybridize to Mu9 are abundant in active Mutator lines, but they are absent from lines that have epigenetically lost Mutator activity. These correlations suggest that Mu9 is a candidate for the autonomous Mutator element | ||
| 546 | _aEnglish | ||
| 595 | _aAC | ||
| 650 | 1 | 0 | _aCarbohydrates |
| 650 | 1 | 0 | _aCell structure |
| 650 | 1 | 0 | _aChromosomes |
| 650 | 1 | 0 | _aGlycosides |
| 650 | 1 | 0 | _aGramineae |
| 650 | 1 | 0 | _aNucleic compounds |
| 650 | 1 | 0 | _aNucleus |
| 650 | 1 | 7 |
_aPlant genetics and breeding _gNOT IN AGROVOC _2 _91208 |
| 650 | 1 | 0 | _aPlant physiology and biochemistry |
| 650 | 1 | 0 | _aZea |
| 650 | 1 | 0 |
_91130 _aGenetics _gAGROVOC |
| 700 | 1 |
_aWalbot, V., _ecoaut. _9364 |
|
| 700 | 1 |
_aWarren, C.A., _ecoaut. |
|
| 773 | 0 | _tProceedings of the National Academy of Sciences of the United States of America (USA). (15 Nov 1991). v. 88(22) p. 10198-10202 | |
| 942 | _cJA | ||
| 999 |
_c22731 _d22731 |
||