000			02803nab a22004697a 4500
001			G448251
003			MX-TxCIM
005			20240919021144.0
008			121211b |||p||p||||||| |z||| |
022			_a0721-7714
022			_a1432-203X (Online)
024			_2https://doi.org/10.1007/s002990050358
040			_aMX-TxCIM
041			_aeng
072		0	_aF30
072		0	_aH10
090			_aCIS-3435
100	1		_aPellegrineschi, A. _96676
245	1	0	_aIn vitro plant regeneration via organogenesis of cowpea [Vigna unguiculata (L.) Walp.].
260			_c1997. _aBerlin (Germany) : _bSpringer,
340			_aPrinted
520			_aShoot regeneration via organogenesis was achieved from axenic cowpea [Vigna unguiculata subsp. unguiculata L. (Walp.) Verde.] hypocotyls and cotyledons of advanced breeding lines and varieties. Cotyledons and embryos were excised from green immature pods. The apical parts of the embryos were removed and the hypocotyls were transferred to regeneration media. Cotyledons and hypocotyls were tested on media with gradients of several hormonal and putrescine combinations. Cowpea cotyledons and hypocotyls exhibited a pattern of shoot formation that occurred in three distinct phases. Multiple shoots developed within 45 days from the wounded region of the primary hypocotyl and cotyledons in different media containing a high cytokinin concentration. The induced plant explants were then grown for 20 days in low-intensity light (10 µmol m–2 s–1) on the same medium and numerous shoot buds emerged de novo from the upper part of the hypocotyl and the wounded part of the cotyledons. These buds had no apparent vascular connection with the parent tissues. The plant regeneration capability of this procedure was tested with several cowpea genotypes, five of which (83D-442, 86D-1010, 93K-624, Vita 3 and Ife Brown) responded positively with shoot development and were able to form roots and whole plants. Some somaclonal variation was observed.
546			_aText in English
591			_a0209|Springer|AL-ABC Program|R01JOURN
595			_aCSC
650	1	7	_aCotyledons _931558 _gAGROVOC
650	1	0	_aCowpeas
650	1	7	_aCowpeas _2AGROVOC _95144
650	1	7	_aCrops _2AGROVOC _91069
650	1	7	_aExplants _2AGROVOC _917330
650	1	7	_91150 _aHormones _2AGROVOC
650	1	7	_aHypocotyls _2AGROVOC _931559
650	1	7	_aOrganogenesis _2AGROVOC _911811
650	1	7	_aPlants _2AGROVOC _94199
650	1	7	_aRegeneration _2AGROVOC _913484
650	1	7	_aSomaclonal variation _91271 _2AGROVOC
650	1	7	_aVigna unguiculata _2AGROVOC _911957
650	1	7	_91134 _aGenotypes _2AGROVOC
650	1	7	_aPlant breeding _gAGROVOC _2 _91203
773	0		_tPlant Cell Reports _gv. 17, p. 89-95 _dBerlin (Germany) : Springer, 1997. _wu79234 _x0721-7714
942			_cJA _2ddc
999			_c22456 _d22456