000 03558nab a22005177a 4500
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022 _a1436-8730
022 _a1522-2624 (Online)
024 _2https://doi.org/10.1002/1522-2624(200206)165:3<357::AID-JPLN357>3.0.CO;2-V
040 _aMX-TxCIM
041 _aeng
072 0 _aF08
072 0 _aF60
090 _aCIS-3397
100 1 _aCollet, L.
_931555
245 1 0 _aAssessment of aluminum sensitivity of maize cultivars using roots of intact plants and excised root tips
260 _c2002.
_aGermany :
_bWiley,
340 _aPrinted
500 _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1436-8730
520 _aMaize cultivars (Zea mays L.) were evaluated for their aluminum (Al) sensitivity using intact plants and excised root tips exposed to 25 μM Al in nutrient solution of low ionic strength and pH 4.3. Aluminum supply increased callose formation and Al concentrations in root tips of intact plants as well as in excised root tips. Using intact plants, differences in Al sensitivity among cultivars could be characterized by Al-induced callose formation, Al-induced inhibition of root elongation, as well as Al contents in root tips as parameters. Significant correlations between Al-induced callose formation and Al contents in root tips (r2 = 0.64**) and inhibition of root elongation (r2 = 0.80***) were found. Excised root tips did not show a significant Al-induced inhibition of root elongation. While average Al-induced callose formation was similar for root tips of intact plants and excised root tips, mean Al contents in excised root tips were up to 1.5-fold higher than in root tips of intact plants after 24 h of Al treatment. Aluminum-induced callose formation as found in excised root tips did neither correspond to Al-induced callose formation nor to inhibition of root elongation of intact plants. The addition of 10 mM glucose to the incubation medium led to a significant increase in the elongation of excised root tips and a 2-3-fold increase in Al-induced callose formation. Staining with triphenyl-tetrazolium-chloride (TTC) revealed increased viability of these root segments. However, these effects of glucose supply did not improve the characterization of the cultivars for Al resistance. The results presented suggest that Al exclusion mechanisms expressed in root tips of intact plants might be non-operational in excised root tips. Therefore, the characterization of maize germplasm for Al resistance using excised root tips appears not to be reliable.
546 _aText in English
591 _aJohn Wiley
592 _aDE-UHa 2001 COLLET D r
595 _aCSC
650 1 7 _aAluminium
_2AGROVOC
_915626
650 1 7 _aCultivation
_2AGROVOC
_91071
650 1 7 _aGlucose
_2AGROVOC
_922525
650 1 7 _aMaize
_2AGROVOC
_91173
650 1 7 _aNutrient solutions
_2AGROVOC
_931554
650 1 7 _aPlant nutrition
_2AGROVOC
_95226
650 1 7 _91755
_aRoots
_2AGROVOC
650 1 7 _aToxic substances
_2AGROVOC
_920589
650 1 7 _91314
_aZea mays
_2AGROVOC
650 1 7 _91134
_aGenotypes
_2AGROVOC
700 1 _aDe Leon, C.
_96688
700 1 _aKollmeier, M.
_931556
700 1 _aSchmohl, N.
_931557
700 1 _aHorst, W.J.
_924535
740 _a448100
740 _a75845
773 0 _tJournal of Plant Nutrition and Soil Science
_gv. 165, no. 3, p. 357-365
_dGermany : Wiley, 2002.
_x1522-2624
942 _cJA
_2ddc
999 _c22441
_d22441