| 000 | 02304nab a22003977a 4500 | ||
|---|---|---|---|
| 001 | G74805 | ||
| 003 | MX-TxCIM | ||
| 005 | 20211006080522.0 | ||
| 008 | 121211b |||p||p||||||| |z||| | | ||
| 022 | 0 | _a0026-8925 | |
| 040 | _aMX-TxCIM | ||
| 082 | 0 | 4 | _a94-064801 |
| 100 | 1 | _aWu, K.S. | |
| 245 | 0 | 0 | _aAbundance, polymorphism and genetic mapping of microsatellites in rice |
| 260 | _c1993 | ||
| 340 | _aPrinted | ||
| 500 | _a4 graphs, 6 tables; 45 ref. Summary (En) | ||
| 520 | _aDinucleotide microsatellites have been characterized and used as genetic markers in rice. Screening of a rice genomic library with poly(dG-dA)/(dC-dT) and poly(dG-dT)/(dC-dA) probes indicated that (GA)(n) repeats occurred, on average, once every 225 kb and (GT)(n) repeats once every 480 kb. DNA sequencing of ten randomly selected microsatellites indicated that the numbers of repeats ranged from 12 to 34 and that the patterns of microsatellites in rice were similar to those of humans and other mammals. Primers to these microsatellite loci as well as to four published microsatellite-containing sequences have been designed and degrees of polymorphism has been examined with 20 rice accessions. Multiple alleles, ranging from 5 to 11, have been observed at all the microsatellite loci in 20 rice accessions. Alleles specific to two cultivated subspecies, indica and japonica, were found in some microsatellite loci. Heterozygosity values of all the microsatellite markers were significantly higher than those of RFLP markers, based upon a parallel comparison. Ten microsatellite loci have been genetically mapped to four rice chromosomes. The genomic distribution of microsatellites appears to be random in rice | ||
| 546 | _aEnglish | ||
| 595 | _aAC | ||
| 650 | 1 | 0 | _aAcids |
| 650 | 1 | 0 | _aCytogenetics |
| 650 | 1 | 7 |
_aGenetic variation _gAGROVOC _2 _91129 |
| 650 | 1 | 7 |
_aGenomes _gAGROVOC _2 _91131 |
| 650 | 1 | 0 | _aGramineae |
| 650 | 1 | 0 | _aNucleic acids |
| 650 | 1 | 0 | _aNucleic compounds |
| 650 | 1 | 0 | _aOrganic acids |
| 650 | 1 | 0 | _aOryza |
| 650 | 1 | 7 |
_aPlant genetics and breeding _gNOT IN AGROVOC _2 _91208 |
| 650 | 1 | 0 | _aPolymorphism |
| 650 | 1 | 0 |
_91130 _aGenetics _gAGROVOC |
| 700 | 1 |
_aTanksley, S.D., _ecoaut. |
|
| 773 | 0 | _tMolecular and General Genetics (Germany). (1993). v. 241(1-2) p. 225-235 | |
| 942 | _cJA | ||
| 999 |
_c22058 _d22058 |
||