000 02424nab a22004337a 4500
001 G73954
003 MX-TxCIM
005 20211006080519.0
008 121211b |||p||p||||||| |z||| |
022 0 _a0036-5378
040 _aMX-TxCIM
082 0 4 _a95-098566
100 1 _aMachan, F.
245 0 0 _aProduction of haploid plants of new wheat and oat donors through wheat x maize and oat x maize crosses
260 _c1995
340 _aPrinted
500 _a1 graph, 4 ill., 2 tables; 21 ref. Summaries (Cs, En)
520 _aStabilization and homogenization of genetic properties of wheat and oats using this method is based on wide crosses of chosen genetic resources of wheat with maize, and oat with maize. The major purpose was to verify a method for obtaining the growing embryos after wide crosses, and to test suitable biotechnological procedures for in vitro cultivating embryos and subsequent dihaploidization using colchicine. Among three series of wide crosses various amounts of embryos from pollinated florets were obtained. The frequency of embryos obtained ranged from 0 to 48 %. The possibility of obtaining embryos from crosses of intact plant and from crosses on detached stems (EDS), and usability of female components obtained in the field or cultivated in the glasshouse were also evaluated. Extirpation and in vitro cultivation of haploid embryos, their cytogenetic control, subsequent dihaploidization and plant cultivation till maturity showed that this method was effective for rapid developing genetically homogeneous materials. It was confirmed that successful solving and especially the frequency of embryos depended on accurate handling the plant materials and growth media used
546 _aEnglish
595 _aAC
650 1 0 _aAvena
650 1 0 _aBiological properties
650 1 7 _aBreeding methods
_gAGROVOC
_2
_91030
650 1 0 _aCell structure
650 1 0 _aChromosome number
650 1 0 _aCulture techniques
650 1 0 _aExperimentation
650 1 0 _aGramineae
650 1 0 _aIn vitro culture
650 1 0 _aNatural resources
650 1 7 _aPlant genetics and breeding
_gNOT IN AGROVOC
_2
_91208
650 1 0 _aProgeny
650 1 0 _aZea
650 1 7 _aTriticum
_gAGROVOC
_2
_91295
700 1 _aNesvadba, Z.,
_ecoaut.
700 1 _aOhnoutkova, L.,
_ecoaut.
773 0 _tGenetika a Slechteni UZPI (Czech Republic). (Jan 1995). v. 31(1) p. 1-10
942 _cJA
999 _c21855
_d21855