| 000 | 02869nab a22004337a 4500 | ||
|---|---|---|---|
| 001 | G73895 | ||
| 003 | MX-TxCIM | ||
| 005 | 20211006080518.0 | ||
| 008 | 121211b |||p||p||||||| |z||| | | ||
| 022 | 0 | _a0027-8424 | |
| 040 | _aMX-TxCIM | ||
| 082 | 0 | 4 | _a93-064076 |
| 100 | 1 | _aStraeten, D. van der | |
| 245 | 0 | 0 | _aCloning, genetic mapping, and expression analysis of an Arabidopsis thaliana gene that encodes 1-aminocyclopropane-1-carboxylate synthase |
| 260 | _c1992 | ||
| 340 | _aPrinted | ||
| 500 | _aill. references US (DNAL 500 N21P) | ||
| 520 | _aA genomic clone of one member of the Arabidopsis thaliana (L.) Heynh. 1-aminocyclopropane-1-carboxylate (ACC) synthase (S-adenosyl-L-methionine methylthioadenosine-lyase, EC 4.4.1.14) gene family (AT-ACC1) was isolated and sequenced. A region of homology was found in the 5'-untranslated region with the promoter of a zucchini and a tomato ACC synthase gene. Comparison of its primary structure with other ACC synthases revealed conservation of seven peptide regions as well as similarity with 11 amino acids of the catalytic site of aminotransferases. Genomic DNA gel blotting suggested the existence of an ACC synthase multigene family in Arabidopsis, possibly with three other members, none of which is very closely related to AT-ACC1. The existence of at least one other gene was confirmed by the isolation of a cDNA (AT-ACC2) from a flower-specific cDNA library. The AT-ACC1 gene was mapped on the Arabidopsis restriction fragment length polymorphism map and is located on the top of chromosome 1. This position does not correspond to any known mutation on the genetic map. Expression of the AT-ACC1 gene was studied by reverse transcription-PCR on total RNA. Messenger accumulation was strong in young leaves and flowers. The gene was not induced by wounding of young leaves or in seedlings in the presence of auxin. Ethylene exposure of mature plants led to an induction of AT-ACC1 gene expression. It is suggested that AT-ACC1 protein has a role in developmental control of ethylene synthesis | ||
| 546 | _aEnglish | ||
| 595 | _aAC | ||
| 650 | 1 | 0 | _aBiochemical reactions |
| 650 | 1 | 0 | _aCell structure |
| 650 | 1 | 0 | _aChemical reactions |
| 650 | 1 | 0 | _aChromosomes |
| 650 | 1 | 0 | _aEnzymes |
| 650 | 1 | 7 |
_aGenomes _gAGROVOC _2 _91131 |
| 650 | 1 | 0 | _aHydrocarbons |
| 650 | 1 | 0 | _aNucleus |
| 650 | 1 | 7 |
_aPlant genetics and breeding _gNOT IN AGROVOC _2 _91208 |
| 650 | 1 | 0 | _aPlant physiology and biochemistry |
| 650 | 1 | 0 |
_91130 _aGenetics _gAGROVOC |
| 700 | 1 |
_aGoodman, H.M., _ecoaut. |
|
| 700 | 1 |
_aHanley, S., _ecoaut. |
|
| 700 | 1 |
_aMontagu, M. van, _ecoaut. |
|
| 700 | 1 |
_aRodrigues Pousada, R.A., _ecoaut. |
|
| 700 | 1 |
_aVillarroel, R., _ecoaut. |
|
| 773 | 0 | _tProceedings of the National Academy of Sciences of the United States of America (USA). (15 Oct 1992). v. 89(20) p. 9969-9973 | |
| 942 | _cJA | ||
| 999 |
_c21799 _d21799 |
||