000			02823nab a22003617a 4500
001			G70965
003			MX-TxCIM
005			20240124163922.0
008			121211b |||p||p||||||| |z||| |
022			_a0040-5752
022			_a1432-2242 (Online)
024			_2https://doi.org/10.1007/BF00285012
040			_aMX-TxCIM
041			_aeng
090			_aREP-521
100	1		_aLapitan, N.L.V. _930999
245	1	0	_aTranslocations and other karyotypic structural changes in wheat X rye hybrids regenerated from tissue culture
260			_c1984. _aGermany : _bSpringer Verlag,
340			_aPrinted
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
520			_aThe spontaneous occurrence of chromosome breaks, deletions, and translocations in plant tissue cultures is well documented. This study investigated the usefulness of tissue culture as a method of introgressing alien genes into wheat. Wheat X rye hybrids were regenerated from embryo scutellar calli maintained in culture for 222 days. The regenerated seedlings then were treated with colchicine to produce amphidiploids (AABBDDRR). The karyotypes of ten amphidiploids were analyzed by C-banding to determine chromosome structural changes that occurred during tissue culture. Three wheat/rye and one wheat/wheat chromosome translocations, seven deletions, and five amplifications of heterochromatin bands of rye chromosomes were identified. One amphidiploid contained a reciprocal translocation between wheat chromosome 4D and rye chromosome 1R. Non-reciprocal translocations between 2B and 3R, and between an unidentified wheat chromosome and 2R, were found independently in two amphidiploids. An additional plant had a translocation between wheat chromosomes 6B and 5A. All deletions involving rye chromosomes were noted in all 10 amphidiploids. Twelve of the 13 breakpoints in chromosomes involved in translocations and deletions occurred in heterochromatin. Amplification of heterochromatin bands on 2RL and 7RL chromosome arms also was observed in five plants. These results indicate a high degree of chromosome structural change induced by tissue culture. Therefore, tissue culture may be a useful tool in alien gene introgression and manipulation of heterochromatin in triticale improvement.
546			_aText in English
591			_aSpringer
595			_aRPC
650		7	_2AGROVOC _91310 _aWheat
650		7	_2AGROVOC _91151 _aHybrids
650		7	_2AGROVOC _97014 _aChromosomes
650		0	_aTranslocation (plant physiology) _gAGROVOC _91858
700	1		_aSears, R.G. _922791
700	1		_aGill, B.S. _98710
773	0		_tTheoretical and Applied Genetics _gv. 68, no. 6, p. 547-554 _dGermany : Springer Verlag, 1984. _wu444762 _x0040-5752
942			_cJA _2ddc
999			_c20734 _d20734