000			01310nab a22002657a 4500
001			G65243
003			MX-TxCIM
005			20170719110406.0
008			121211b |||p||p||||||| |z||| |
040			_aMX-TxCIM
082	0	4	_a81-687149
100	1		_aDunwell, J.M.
245	0	0	_aStimulation of pollen embryo induction in tobacco by pretreatment of excised anthers in a water-saturated atmosphere
260			_c1980
340			_aPrinted
500			_a11 ref.; Summary (En)
520			_aThe effect on the embryogenic potential of tobacco anthers of a pre-culture period in a water-saturated atmosphere was examined. It was found that maintaining excised anthers in such an atmosphere for 2 or 3 days at 23 deg C increased the number of anthers subsequently producing pollen plants 4-fold and doubled the number in which embryos were induced. This increase in frequency was negatively correlated with the rate of anther senescence in culture. It is suggested that some component of the usual culture medium is toxic to anthers during the early period of culture
546			_aEnglish
595			_aAC
650	1	0	_aPlant physiology and biochemistry
650	1	0	_aPollen
650	1	0	_aTobacco nicotiana spp.
773	0		_tPlant Science Letters (Netherlands). (Mar 1980). v. 21(1) p. 9-13
942			_cJA
999			_c18695 _d18695