000 02515nab a22004577a 4500
001 G63067
003 MX-TxCIM
005 20171220113519.0
008 121211b |||p||p||||||| |z||| |
022 _a0974-1275 (Revista en electrónico)
022 0 _a0971-7811
024 8 _ahttps://doi.org/10.1007/BF03321955
040 _aMX-TxCIM
041 0 _aEn
072 0 _aF30
072 0 _aH20
082 0 4 _a95-033587
100 1 _9892
_aSukhwinder-Singh
_gGenetic Resources Program
_8INT3098
245 0 0 _aTowards molecular tagging of karnal but resistance gene(s) in wheat
260 _c1994
340 _aPrinted
500 _aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0971-7811
520 _aWe describe preliminary results of cosegregation of a DNA probe with Karnal bunt resistance genets) in wheat. The tagging strategy utilized 4 uniformly resistant and 13 uniformly susceptible F3 families from a cross of Karnal bunt resistant (HD29) and susceptible (WL711) lines of Triticum aestivum. Bulked DNA of 8?10 plants in each family were tested by gel blot DNA hybridization. Thirty three per cent of the probes (11/33) detected restriction fragment length polymorphism (RFLP) between Karnal bunt resistant and susceptible lines. Probe Cxp1 from chromosome group 6 detected the resistant parent (HD29) polymorphic band in all the resistant family bulks but was missing in 6 uniformly susceptible family bulks. A similar pattern was observed with probe XKsu G34 from 1DL. The intensity of the resistant parent band in the susceptible family bulks was less as compared to the resistant family bulks. Probe Xksu H8 which mapped on chromosomes 2, 3, 5 and 7D on the T. tauschii map did not differentiate resistant family bulks from susceptible family bulks. Our results indicated that one or more resistance genets) are located on chromosome groups 1 and 6 of wheat.
536 _aGenetic Resources Program
546 _aEnglish
591 _aNo CIMMYT affiliation|Springer
594 _aINT3098
595 _aAC
595 _aRPC
650 1 0 _aBulk family analysis
650 1 0 _aKamal bunt
650 1 0 _aRFLP
650 1 0 _aT. aestivum
650 1 0 _aWheat
700 1 _aDhaliwal, H.S.,
_ecoaut.
700 1 _aGill, B.S.,
_ecoaut.
700 1 _aGill, K.S.,
_ecoaut.
700 1 _aSingh, H.,
_ecoaut.
773 0 _tJournal of Plant Biochemistry and Biotechnology
_n95-033587
_gv. 3, no. 2, p. 79-83
942 _cJA
999 _c18326
_d18326