000			02183nab a22003857a 4500
999			_c16812 _d16812
001			G56569
003			MX-TxCIM
005			20211006072023.0
008			121211b |||p||p||||||| |z||| |
022			_a1432-2242 (Revista en electrónico)
040			_aMX-TxCIM
041	0		_aEn
043			_aNL
072		0	_aF30
090			_aLook _bunder journal title
100	1		_aFaridi, N.I.
245	0	0	_aVisualization of Secale cereale DNA in wheat germ plasm by fluorescent in situ hybridization
260			_c1995
340			_aPrinted
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
520			_aHomozygous wheat/rye (1BL/1RS or 1AS/1RL) translocation lines have significantly contributed to wheat production, and several other wheat/rye translocation lines show a potential promise against biotic and abiotic stresses. Detecting the presence of rye at the chromosome level is feasible by C-banding and isozyme protocols, but the diagnostic strength of genomic in situ hybridization for eventually analysing smaller DNA introgressions has greater significance. Non-radioactive in situ hybridization using labelled total genomic DNA of alien species was successfully utilized with the unlabelled blocking DNA of T. aestivum and T. durum. By this method rye contributions to the translocations 1Bll/1RS, 1AL/1RS, 5AS/5RL and 6BS/6RL could be identified. Differential labelling has further enabled the detection of rye and Thinopyrum bessarabicum [Elymus bessarabicus] chromosomes in a trigeneric hybrid of Triticum aestivum/T. bessarabicum/Secale cereale
546			_aEnglish
591			_aWP|Springer|R95ANALY|1
650	1	0	_aChromosome translocation
650	1	0	_aDNA
650	1	0	_aHybridization
650	1	0	_aResearch projects _91237
650	1	0	_aSecale cereale
650	1	7	_aWheat _gAGROVOC _2 _91310
653	0		_aCIMMYT
700	1		_aMujeeb-Kazi, A., _ecoaut. _9221
773	0		_tTheoretical and Applied Genetics _n999465 _gv. 90, no. 5, p. 595-600
856	4		_yAccess only for CIMMYT Staff _uhttp://libcatalog.cimmyt.org/download/cis/G56569.pdf
942			_cJA _2ddc _n0