000			02252nab a22003977a 4500
001			G53266
003			MX-TxCIM
005			20211006072019.0
008			121211b |||p||p||||||| |z||| |
022			_a1573-5060 (Revista en electrónico)
040			_aMX-TxCIM
041	0		_aEn
043			_aNL
072		0	_aF02
072		0	_aF63
090			_aREFERENCE _bONLY
100	1		_aImmonen, A.S.T.
245	0	0	_aComparison of callus culture with embryo culture at different times of embryo rescue for primary triticale production
260			_c1993
340			_aPrinted
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0014-2336
520			_aCallus culture of immature wheat-rye hybrid embryos was compared with embryo culture in 2 experiments. Embryos were rescued from field grown female plants at 2 day intervals 13-21 days after pollination and (1) cultured on MS medium supplemented with 2 mg 2,4-D/litre followed by plant regeneration on hormone free MS medium with half strength mineral salts, and (2) cultured on Taira and Larter medium. Observations were made on embryo size and condition at time of rescue (experiment 1) and embryo development directly into plants (embryo culture) or through somatic embryogenesis (callus culture). Fewer 19 and 21-day-old embryos developed into plants from callus culture than from embryo culture in experiment 1. Callus culture was more efficient than embryo culture in promoting plant recovery from 17-day-old embryos in experiment 2. The number of plants per embryo was significantly higher from callus culture than from embryo culture. In both experiments callus culture promoted embryogenesis in more embryos than developed in embryo culture. Embryo rescue 15-17 days after pollination was optimal in both experiments
546			_aEnglish
591			_aSpringer
592			_aFI-HY 1996 IMMONEN D r
650	1	0	_aCallus
650	1	0	_aEmbryo culture
650	1	0	_aSecale cereale
650	1	0	_aTissue culture _91291
650	1	0	_aTriticales _91294
650	1	7	_aWheat _gAGROVOC _2 _91310
740			_a53266
740			_a92756
773	0		_tEuphytica _n999611 _gv. 70, no. 3, p. 185-190
942			_cJA
999			_c15975 _d15975