000			01712nab a22004097a 4500
001			G51218
003			MX-TxCIM
008			121211b |||p||p||||||| |z||| |
022	0		_a1050-3862
040			_aMX-TxCIM
041	0		_aEn
043			_aUS
072		0	_aF30
082	0	4	_a93-074116
100	1		_aShattuck Eidens, D.M.
245	0	0	_aRapid detection of maize DNA sequence variation
260			_c1991
340			_aPrinted
520			_aThe allele-specific polymerase chain reaction (ASPCR) has been used to determine the genotype of maize lines at two loci, wx and NPI288. The ASPCR method uses allele-specific oligonucleotide primers in PCR amplifications to amplify and discriminate simultaneously between polymorphic alleles. The success of this technique relies on the specific failure of PCR to amplify, with primers that do not perfectly match the DNA sequence of one of the allelic variants. Amplification results were evaluated by dot-blot hybridization using an alkaline-phosphatase-coupled probe. The technique's speed, accuracy sensitivity and high throughput make it
546			_aEnglish
591			_aUS (DNAL QH442.G446)
595			_aAC
650	1	0	_aBiochemistry _91021
650	1	0	_aDNA
650	1	0	_aGenes
650	1	0	_91848 _aGenetic markers _gAGROVOC
650	1	7	_aGenetic variation _gAGROVOC _2 _91129
650	1	0	_91172 _aLoci _gAGROVOC
650	1	0	_aNucleotides
650	1	0	_91314 _aZea mays _gAGROVOC
650	1	0	_91134 _aGenotypes _gAGROVOC
700	1		_aBell, R.N., _ecoaut.
700	1		_aMcWhorter, V.C., _ecoaut.
700	1		_aMitchell, J.T., _ecoaut.
773	0		_tGene analysis techniques and applications _n93-074116 _gv. 8, no. 8, p. 240-245
942			_cJA
999			_c15899 _d15899