000			01397nab a22003017a 4500
001			G49866
003			MX-TxCIM
008			121211b |||p||p||||||| |z||| |
040			_aMX-TxCIM
041	0		_aEn
043			_aNL
072		0	_aF30
082	0	4	_a82-765572
100	1		_aKarunaratne, S.M.
245	0	0	_aMitotic activity in protoplasts isolated from Sorghum bicolor leaves
260			_c1981
340			_aPrinted
500			_a7 ref.; Summary (En)
520			_aFactors affecting viability and mitotic activity of mesophyll protoplasts of Sorghum bicolor have been investigated. Protoplasts were isolated from leaves of 7-day-old seedlings which had been kept in continuous darkness for 3 days prior to enzymatic digestion. Subsequent incubation of these protoplasts in a medium conditioned by prior growth of sorghum shoot callus resulted in over 90 per cent of the original population remaining viable after 1 day and 5.2 per cent undergoing nuclear division after 17 days. Also, [('3)H]-thymidine incorporation and autoradiography confirmed that DNA synthesis was occurred in these protoplasts. Nuclear division
546			_aEnglish
591			_aCOMOD
595			_aAC
650	1	0	_aSorghums sorghum spp
650	1	7	_aPlant breeding _gAGROVOC _2 _91203
700	1		_aScott, K.J., _ecoaut.
773	0		_tPlant Science Letters _n82-765572 _gv. 23, no. 1, p. 11-16
942			_cJA
999			_c14588 _d14588