000			01296nab a22003257a 4500
001			G49850
003			MX-TxCIM
008			121211b |||p||p||||||| |z||| |
040			_aMX-TxCIM
041	0		_aEn
043			_aNL
072		0	_aF00
072		0	_aF60
082	0	4	_a82-763994
100	1		_aGaspar, S.
245	0	0	_aA new method for tetrazolium analysis of wheat seeds without embryo excision
260			_c1981
340			_aPrinted
500			_a5 ref.; Summaries (De, En, Fr)
520			_aWe estimated the viability of 20 wheat seed samples without embryo excision. After soaking in water the seeds were immersed in tetrazolium solution and treated by ultrasound frequency 22 kHz, intensity 0.69 W/cm('2) for from five to 10 minutes. The ultrasound treatment promoted penetration into imbibed seeds. After 24 hours staining at 20 deg C the seeds were boiled in lactophenol to clear the second coat. The viability of embryos was evaluated through the seed coat, without separation of the embryo
546			_aEnglish
591			_aCOMOD
595			_aAC
650	1	0	_aPlant production _91212
650	1	0	_aSeeds
650	1	0	_aWheat triticum spp.
700	1		_aNagy, J., _ecoaut.
773	0		_tSeed Science and Technology _n82-763994 _gv. 9, no. 2, p. 553-556
942			_cJA
999			_c14574 _d14574