000			01509nab a22003137a 4500
001			G49522
003			MX-TxCIM
008			121211b |||p||p||||||| |z||| |
040			_aMX-TxCIM
041	0		_aEn
043			_aNL
072		0	_aF60
082	0	4	_a81-663982
100	1		_aM'Voula Tsieri, M.
245	0	0	_aProperties of nucleosides diphosphatases in purified membrane fractions from maize coleoptiles, 1: _b study of latency
260			_c1981
340			_aPrinted
500			_a18 ref.; Summary (En)
520			_aEvidence is given for the presence of nucleoside diphosphatase (NDPase) activities in two membrane fractions from maize coleoptiles, one enriched in endoplasmic reticulum (ER) and the other in plasma membranes (PM). The NDPase activity associated with the ER-rich fraction appears only after treatment of membranes with detergent. Enzyme inactivation experiments using trypsin were performed on intact and Triton X-100-treated vesicles. They suggest that in the endoplasmic reticulum-rich fraction, most of enzymatic sites have a lumenal location and that in plasma membrane vesicles, only 40-50 per cent of enzymatic sites are not freely accessible to nucleosides
546			_aEnglish
591			_aCOMOD
595			_aAC
650	1	7	_aMaize _gAGROVOC _2 _91173
650	1	0	_91314 _aZea mays _gAGROVOC
700	1		_aBenveniste, P., _ecoaut.
700	1		_aHartmann Bouillon, M.A., _ecoaut.
773	0		_tPlant Science Letters _n81-663982 _gv. 20, no. 4, p. 379-386
942			_cJA
999			_c14259 _d14259