000			01436nab a22003137a 4500
001			G49297
003			MX-TxCIM
008			121211b |||p||p||||||| |z||| |
040			_aMX-TxCIM
041	0		_aEn
043			_aNL
072		0	_aL36
082	0	4	_a81-589049
100	1		_aEl Sherbiny, A.E.
245	0	0	_aThe determination of available lysine in barley using Tetrahymena pyriformis W
260			_c1980
340			_aPrinted
500			_a11 ref.; Summary (En)
520			_aAttempts to use the microbiological procedure based on growth of Tetrahymena for the assay of available lysine in barley led to several technical problems. These were mainly due to interference by carbohydrate with the measurement of the growth response of the organism, either by cell counting or by optical density. The assay was modified to suit the measurement of available lysine in barley grains. Fine grinding of samples, predigestion with papain and selection of appropriate N-concentrations in the medium were found to be key factors in adapting the technique for barley. The availability of lysine in barley, as assessed by the modified
546			_aEnglish
591			_aCOMOD
595			_aAC
650	1	0	_aBarley hordeum spp
650	1	0	_aFeed composition
700	1		_aDraper, S.R., _ecoaut.
700	1		_aTopps, J.H., _ecoaut.
773	0		_tAnimal Feed Science and Technology _n81-589049 _gv. 5, no. 1, p. 33-42
942			_cJA
999			_c14046 _d14046