000 01539nab a22003377a 4500
001 G33622
003 MX-TxCIM
008 121211b |||p||p||||||| |z||| |
040 _aMX-TxCIM
041 0 _aEn
043 _aNL
072 0 _aH20
090 _aREP-3724
100 1 _aJaegle, M.
245 0 0 _aUse of ELISA for measuring the extent of serological cross-reactivity between plant viruses
260 _c1985
340 _aPrinted
500 _aTables, graphs, references p. 198
520 _aThe degree of antigenic relatedness between 2 plant viruses is commonly expressed by a serological differentiation index (SDI) which corresponds to the av. number of 2-fold dilution steps separating homologous from heterologous precipitin titres. Results obtained with several tobamo- and tombusviruses indicated that the indirect form of ELISA can also be used for calculating SDI values. This was achieved by comparing the antiserum dilutions that lead to the same absorbance measurements when homologous and heterologous viruses are assayed by ELISA. SDI values calculated from ELISA were similar to those obtained from precipitin tests. Because of its
546 _aEnglish
595 _aRPC
650 1 0 _aAntigen antibody reactions
650 1 0 _aElisa
650 1 0 _aMeasurement
650 1 0 _aMethods
_91178
650 1 0 _aPlant pathology
_91209
650 1 0 _aPlant viruses
_91543
700 1 _aRegenmortel, M.H.V. Van,
_ecoaut.
773 0 _tJournal of Virological Methods
_n617580
_gv. 11, no. 3, p. 189-198
942 _cJA
999 _c12196
_d12196