000 | 01539nab a22003377a 4500 | ||
---|---|---|---|
001 | G33622 | ||
003 | MX-TxCIM | ||
008 | 121211b |||p||p||||||| |z||| | | ||
040 | _aMX-TxCIM | ||
041 | 0 | _aEn | |
043 | _aNL | ||
072 | 0 | _aH20 | |
090 | _aREP-3724 | ||
100 | 1 | _aJaegle, M. | |
245 | 0 | 0 | _aUse of ELISA for measuring the extent of serological cross-reactivity between plant viruses |
260 | _c1985 | ||
340 | _aPrinted | ||
500 | _aTables, graphs, references p. 198 | ||
520 | _aThe degree of antigenic relatedness between 2 plant viruses is commonly expressed by a serological differentiation index (SDI) which corresponds to the av. number of 2-fold dilution steps separating homologous from heterologous precipitin titres. Results obtained with several tobamo- and tombusviruses indicated that the indirect form of ELISA can also be used for calculating SDI values. This was achieved by comparing the antiserum dilutions that lead to the same absorbance measurements when homologous and heterologous viruses are assayed by ELISA. SDI values calculated from ELISA were similar to those obtained from precipitin tests. Because of its | ||
546 | _aEnglish | ||
595 | _aRPC | ||
650 | 1 | 0 | _aAntigen antibody reactions |
650 | 1 | 0 | _aElisa |
650 | 1 | 0 | _aMeasurement |
650 | 1 | 0 |
_aMethods _91178 |
650 | 1 | 0 |
_aPlant pathology _91209 |
650 | 1 | 0 |
_aPlant viruses _91543 |
700 | 1 |
_aRegenmortel, M.H.V. Van, _ecoaut. |
|
773 | 0 |
_tJournal of Virological Methods _n617580 _gv. 11, no. 3, p. 189-198 |
|
942 | _cJA | ||
999 |
_c12196 _d12196 |