000 | 01431nab a22003257a 4500 | ||
---|---|---|---|
001 | G33617 | ||
003 | MX-TxCIM | ||
008 | 121211b |||p||p||||||| |z||| | | ||
040 | _aMX-TxCIM | ||
041 | 0 | _aEn | |
043 | _aCA | ||
072 | 0 | _aH20 | |
090 | _aREP-3644 | ||
100 | 1 | _aLin, C.P. | |
245 | 0 | 0 | _aMonoclonal antibodies against corn stunt spiroplasma |
260 | _c1985 | ||
340 | _aPrinted | ||
500 | _aTables, references p. 903-904 | ||
520 | _aAntibodies were produced by fusing NS-1/1-Ag4-1 murine myeloma cells with splenic cells from mice immunized with maize stunt spiroplasma (I-747). Isotypeswere determined to be IgG1 or IgG2a subclass. Antibody titres were 800-3200 for hybridoma culture supernatants and 312 500 - 7 812 400 for ascitic fluids. In serological specificity studies, with ELISA, 32 spiroplasma strs. belonging to 19 different serogroups were tested using both maize stunt spiroplasma monoclonal antibodies and conventionally produced polyclonal antibodies. Maize stunt spiroplasma specific monoclonal antibodies reacted specifically with any of the 3 maize stunt | ||
546 | _aEnglish | ||
595 | _aRPC | ||
650 | 1 | 0 | _aElisa |
650 | 1 | 0 | _aIdentification |
650 | 1 | 0 | _aImmunofluorescence |
650 | 1 | 0 | _aMonoclonal antibodies |
650 | 1 | 0 | _aMycoplasmatales |
700 | 1 |
_aChen, T.A., _ecoaut. |
|
773 | 0 |
_tCanadian Journal of Microbiology _n617574 _gv. 31, no. 10, p. 900-904 |
|
942 | _cJA | ||
999 |
_c12191 _d12191 |