000 01431nab a22003257a 4500
001 G33617
003 MX-TxCIM
008 121211b |||p||p||||||| |z||| |
040 _aMX-TxCIM
041 0 _aEn
043 _aCA
072 0 _aH20
090 _aREP-3644
100 1 _aLin, C.P.
245 0 0 _aMonoclonal antibodies against corn stunt spiroplasma
260 _c1985
340 _aPrinted
500 _aTables, references p. 903-904
520 _aAntibodies were produced by fusing NS-1/1-Ag4-1 murine myeloma cells with splenic cells from mice immunized with maize stunt spiroplasma (I-747). Isotypeswere determined to be IgG1 or IgG2a subclass. Antibody titres were 800-3200 for hybridoma culture supernatants and 312 500 - 7 812 400 for ascitic fluids. In serological specificity studies, with ELISA, 32 spiroplasma strs. belonging to 19 different serogroups were tested using both maize stunt spiroplasma monoclonal antibodies and conventionally produced polyclonal antibodies. Maize stunt spiroplasma specific monoclonal antibodies reacted specifically with any of the 3 maize stunt
546 _aEnglish
595 _aRPC
650 1 0 _aElisa
650 1 0 _aIdentification
650 1 0 _aImmunofluorescence
650 1 0 _aMonoclonal antibodies
650 1 0 _aMycoplasmatales
700 1 _aChen, T.A.,
_ecoaut.
773 0 _tCanadian Journal of Microbiology
_n617574
_gv. 31, no. 10, p. 900-904
942 _cJA
999 _c12191
_d12191