000			01620nab a22003737a 4500
001			G28857
003			MX-TxCIM
008			121211b |||p||p||||||| |z||| |
040			_aMX-TxCIM
041	0		_aEn
043			_aDE
072		0	_aF30
072		0	_aF60
090			_aREP-4391
100	1		_aKirihara, J.A.
245	0	0	_aDifferential expression of a gene for a methionine-rich storage protein in maize
260			_c1988
340			_aPrinted
500			_aIllustrations, tables, references p. 483-484
520			_aOn the basis of the sequence of the N-terminal 30 amino acids of a 10 kD methionine-rich zein protein, 2 mixed oligonucleotides were synthesized and usedto probe a maize endosperm cDNA library. A full-length cDNA clone encoding the 10 kD zein was isolated;its nucleotide sequence predicted a polypeptide of 129 amino acids, preceded by a signal peptide of 21 amino acids. The predicted polypeptide was unique in its extremely high content of methionine (22.5%). The inbred line BSSS53, which has increased seed methionine, was compared to W23, a standard inbred line. Northern blot analysis showed that the relative RNA levelsfor the 10 kD zein were enhanced
546			_aEnglish
595			_aRPC
650	1	0	_aMethionine
650	1	0	_aProlamines
650	1	0	_aSeed
650	1	0	_aSeeds
650	1	0	_91314 _aZea mays _gAGROVOC
650	1	0	_91314 _aZea mays _gAGROVOC
700	1		_aHunsperger, J.P., _ecoaut.
700	1		_aMahoney, W.C., _ecoaut.
700	1		_aMessing, J.W., _ecoaut.
773	0		_tMolecular and General Genetics _n615038 _gv. 211, no. 3, p. 477-484
942			_cJA
999			_c11976 _d11976