000 01640nab a22004097a 4500
001 G28802
003 MX-TxCIM
008 121211b |||p||p||||||| |z||| |
040 _aMX-TxCIM
041 0 _aEn
043 _aJP
072 0 _aH20
090 _aREP-3671
100 1 _aMatsubara, A.
245 0 0 _aPurification and serology of a Japanese isolate of barley yellow dwarf virus
260 _c1985
340 _aPrinted
500 _aIllustrations, tables, literature cited p. 157
520 _aBYDV was purified from infected barley plants by an improved procedure whichyielded 44 æg virus/100 g tissues. The isolate had isometric particles, diam. 27.3 nm and a UV absorbance spectrum with an av. A260/A280 ratio of 1.71. In ELISA using the double antibody sandwich method, the virus reacted with homologous antiserum but not with antiserum to potato leafroll virus and vice versa. It reacted slightly with heterologous antisera to other luteoviruses suchas PLRV, soyabean dwarf and beet western yellows viruses in agar gel double diffusion tests.
546 _aEnglish
595 _aRPC
650 1 0 _aBarley
_91018
650 1 0 _aBarley yellow dwarf virus
_91019
650 1 0 _aElisa
650 1 0 _aImmunology
650 1 0 _aJapan
650 1 0 _aPurification
650 1 0 _aRhopalosiphum
700 1 _aHattori, M.,
_ecoaut.
700 1 _aKawano, S.,
_ecoaut.
700 1 _aKojima, M.,
_ecoaut.
700 1 _aNarita, M.,
_ecoaut.
700 1 _aShikata, E.,
_ecoaut.
700 1 _aUyeda, I.,
_ecoaut.
773 0 _tAnnals of the Phytopathological Society of Japan
_n615284
_gv. 51, no. 2, p. 152-158
942 _cJA
999 _c11932
_d11932