| 000 | 01584nab a22003737a 4500 | ||
|---|---|---|---|
| 001 | G28797 | ||
| 003 | MX-TxCIM | ||
| 005 | 20170719154934.0 | ||
| 008 | 121211b |||p||p||||||| |z||| | | ||
| 040 | _aMX-TxCIM | ||
| 041 | 0 | _aEn | |
| 043 | _aFR | ||
| 072 | 0 | _aF30 | |
| 090 | _aREP-4725 | ||
| 100 | 1 | _aMouras, A. | |
| 245 | 0 | 0 | _aFrom repetitive DNA sequences to single-copy gene mapping in plant chromosomes by in situ hybridization |
| 260 | _c1989 | ||
| 340 | _aPrinted | ||
| 500 | _aIllustrations, tables, references p. 168 | ||
| 520 | _aHaploid leaf segments of Nicotiana plumbaginifolia were transformed with Agrobacterium rhizogenes and actively growing haploid root cultures established.Using protoplasts prepared from these cell populations, the single copy, 1.6 kb Adh (alcohol dehydrogenase) gene and repetitive ribosomal DNA sequences were tentatively mapped by in situ hybridization to chromosomes 1 or 5, and 1B, 6B and 5D respectively in metaphase preparations. The addition of sodium dodecylsulfate (SDS) and polyethylene glycol (PEG) to the probe mixture reduced background noise and allowed longer autoradiographic exposures (12-15 weeks) | ||
| 546 | _aEnglish | ||
| 591 | _aElsevier | ||
| 595 | _aRPC | ||
| 650 | 1 | 0 | _aChromosomes |
| 650 | 1 | 0 | _aDNA |
| 650 | 1 | 0 | _aGenetic maps |
| 650 | 1 | 0 |
_aMolecular Biology _91183 |
| 650 | 1 | 0 | _aProtoplasts |
| 700 | 1 |
_aHorth, M., _ecoaut. |
|
| 700 | 1 |
_aJacobs, M., _ecoaut. |
|
| 700 | 1 |
_aNegrutiu, I., _ecoaut. |
|
| 773 | 0 |
_tPlant Physiology and Biochemistry _n609964 _gv. 27, no. 2, p. 161-168 |
|
| 942 | _cJA | ||
| 999 |
_c11927 _d11927 |
||