| 000 | 01740nab a22004097a 4500 | ||
|---|---|---|---|
| 001 | G28760 | ||
| 003 | MX-TxCIM | ||
| 005 | 20170719154933.0 | ||
| 008 | 121211b |||p||p||||||| |z||| | | ||
| 040 | _aMX-TxCIM | ||
| 041 | 0 | _aEn | |
| 043 | _aDE | ||
| 072 | 0 | _aF30 | |
| 090 | _aREP-4881 | ||
| 100 | 1 | _aLyznik, L.A. | |
| 245 | 0 | 0 |
_aStable transformation of maize: _b The impact of feeder cells on protoplast growth and transformation efficiency |
| 260 | _c1989 | ||
| 340 | _aPrinted | ||
| 500 | _aIllustrations, tables, graphs, references p. 295 | ||
| 520 | _aProtoplast cultures of the F1 hybrid A188 X Black Mexican Sweet were transformed using 2 plasmids, pPUR carrying genes coding for beta-glucuronidase (GUS) and pKAN carryong the gene neo neomycin resistance, by either electroporation or PEG [polyethylene glycol] treatment. Total GUS activity, measured 2 days after transformation, was 5-fold higher in protoplasts cultured on feeder cells compared to those grown in the absence of feeder cells. Since the specific activity of GUS was only slightly higher in the transformed protoplasts plated over feeder cells, the stimulation in transient gene expression resulted mainly from the improved environment | ||
| 546 | _aEnglish | ||
| 591 | _aSpringer | ||
| 595 | _aRPC | ||
| 650 | 1 | 0 | _aAntibiotics |
| 650 | 1 | 0 | _aCell culture |
| 650 | 1 | 0 | _aGenes |
| 650 | 1 | 0 | _aGenetic transformation |
| 650 | 1 | 0 | _aProtoplasts |
| 650 | 1 | 0 |
_91314 _aZea mays _gAGROVOC |
| 700 | 1 |
_aChang, K.L., _ecoaut. |
|
| 700 | 1 |
_aGrimes, H.D., _ecoaut. |
|
| 700 | 1 |
_aHodges, T.K., _ecoaut. |
|
| 700 | 1 |
_aKamo, K.K., _ecoaut. |
|
| 700 | 1 |
_aRyan, R, _ecoaut. |
|
| 773 | 0 |
_tPlant Cell Reports _n615170 _gv. 8, no. 5, p. 292-295 |
|
| 942 | _cJA | ||
| 999 |
_c11896 _d11896 |
||