000			02073nab a22003857a 4500
001			G17776
003			MX-TxCIM
005			20240919021117.0
008			121211b |||p||p||||||| |z||| |
022			_a0009-0352
022			_a1943-3638 (Online)
040			_aMX-TxCIM
041	0		_aeng
072		0	_aF01
072		0	_aF30
090			_aCIS-165
100	1		_aVillegas, E. _93651
245	1	0	_aDetermination of lysine on the automated amino acid analyzer by a triplicate-sample method.
260			_c1968. _aSt. Paul, MN (USA) : _bWiley,
340			_aPrinted
500			_aPeer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0009-0352
520			_aA triplicate-sample method was developed for determination of lysine by ion-exchange chromatography on an automated amino acid analyzer. In this method, three samples of cereal hydrolysate are applied to the short column of the analyzer, and then the lysine from each sample is resolved with pH 5.10 sodium citrate buffer (0.20M in Na) and recorded as a group of three lysine peaks. The resin is next regenerated with 0.2N sodium hydroxide and then equilibrated with citrate buffer for the next triplicate analysis. Accuracy of the lysine analysis by the triplicate-sample method is good. The number of lysine analyses that could be run per day is estimated to be 15 with one column fitted to the analyzer, 24 with two columns, and 36 with three columns. The triplicate-sample method has been used for lysine determination of numerous samples of several cereals.
546			_aText in English
591			_aMIC 8552-R|3
595			_aCSC
650	1	7	_aAmino acids _2AGROVOC _94464
650	1	7	_aCereals _2AGROVOC _91036
650	1	7	_aLysine _2AGROVOC _95257
650	1	7	_aMethods _91178 _2AGROVOC
650	1	7	_aPlant breeding _gAGROVOC _2 _91203
700	1		_aMcDonald, C.E. _97018
700	1		_aGilles, K.A. _97020
773	0		_tCereal Chemistry _gv. 45, no. 5, p. 432-436 _dSt. Paul, MN (USA) : Wiley, 1968. _wG444220 _x1943-3638
942			_cJA _2ddc
999			_c11497 _d11497