000 02881nam a22003737a 4500
001 G89998
003 MX-TxCIM
005 20211006082025.0
008 121211s ||||f| 0 p|p||0|| |
020 _a978-1-4020-5496-9
040 _aMX-TxCIM
090 _aCIS-5055
100 1 _aHays, D.
_uWheat production in stressed environments. Proceedings of International Wheat Conference, 7; Mar de Plata (Argentina); 27 Nov - 2 Dec 2005
245 0 0 _aExpression quantitative trait loci mapping heat tolerance during reproductive development in wheat (Triticum aestivum)
260 _aDordrecht (Netherlands)
_bSpringer :
_c2007
300 _ap. 373-382
340 _aPrinted
490 _aDevelopments in Plant Breeding ;
_v12
520 _aHigh temperature during reproductive development is a major limitation to wheat production and end-use quality in the Southern Great Plains (USA) and to wheat production in many environments worldwide. We have initiated a project to integrate genotypic (QTL), phenotypic and transcript level data to identify genes controlling reproductive stage heat tolerance in heat tolerant genotypes of wheat as it relates to yield and end-use quality maintenance. Efforts have initially focused on building recombinant inbred lines (RILs) and cDNA libraries enriched, through suppressive subtractive hybridization, for genes induced by heat stress. The selected tissues for library construction included wheat heads and flag leaves isolated from plants subjected to heat stress 10 days after pollination. A heat tolerant spring wheat cultivar ‘Halberd’, and a susceptible winter wheat cultivar Cutter were used as models to define the two adaptive responses to heat stress (heat avoidance (susceptible) and heat tolerance). Over 1,920 unique ESTs have been sequenced. These genes include some potential regulatory proteins, heat shock proteins and lipid-transfer proteins, as well as many novel genes that may belong to uncharacterized pathways involved in response to heat stress. For example, a lipid transfer protein and an alpha amylase inhibitor remained stable during heat shock in the heat-tolerant cultivar Halberd. These genes were also highly expressed in the most heat tolerant RILs but not in the most susceptible RILs. Expression-QTL mapping results will be presented which link QTLs controlling heat tolerance to their regulation of discrete sets of the plant transcriptome
536 _aGlobal Wheat Program
546 _aEnglish
593 _aLucia Segura
594 _aINT1511
595 _aCSC
653 0 _aheat tolerance
653 0 _aloci mapping
653 0 _aQTL
700 1 _aBuck, H.T.|Nisi, J.E.|Salomon, N.
_eeds.
700 1 _aHwa Do, J.,
_ecoaut.
700 1 _aMason, E.,
_ecoaut.
700 1 _aMenz, M.,
_ecoaut.
700 1 _aReynolds, M.P.
_gGlobal Wheat Program
_8INT1511
_9831
942 _cPRO
999 _c10225
_d10225