Knowledge Center Catalog

A maop-based approach towards clonning the Yd2 resistance gene in barley

By: Contributor(s): Material type: TextTextPublication details: Mexico, DF (Mexico) CIMMYT : 2002Description: p. 75-77ISBN:
  • 970-648-095-1
Subject(s): DDC classification:
  • 632.3 HEN
Summary: In an attempt to identify and characterize the Yd2 gene, a map-based cloning strategy has been undertaken in our laboratory. Linkage maps in two barley populations have been constructed placing 29 molecular markers, the Yd2 gene, and the centromere on these maps. Twelve of these markers are within 1cM of Yd2. Physical mapping of the Yd2 region revealed a physical linkage between the two loci Xbcd134 and Ylp. Because these loci are separated by O.5cM on the Proctor/Shannon map, a relationship between physical to genetic distance around this immediate area could be calculated as lcM being less than 360kb. Screening of barley bacterial artificial chromosome filters identified a number of Yd2 linked BACs forming a partial contig over the Yd2 region. Further chromosome walks will continue until a complete contig over the Yd2 gene is constructed.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Book CIMMYT Knowledge Center: John Woolston Library CIMMYT Publications Collection 632.3 HEN (Browse shelf(Opens below)) 1 Available V629708
Total holds: 0

In an attempt to identify and characterize the Yd2 gene, a map-based cloning strategy has been undertaken in our laboratory. Linkage maps in two barley populations have been constructed placing 29 molecular markers, the Yd2 gene, and the centromere on these maps. Twelve of these markers are within 1cM of Yd2. Physical mapping of the Yd2 region revealed a physical linkage between the two loci Xbcd134 and Ylp. Because these loci are separated by O.5cM on the Proctor/Shannon map, a relationship between physical to genetic distance around this immediate area could be calculated as lcM being less than 360kb. Screening of barley bacterial artificial chromosome filters identified a number of Yd2 linked BACs forming a partial contig over the Yd2 region. Further chromosome walks will continue until a complete contig over the Yd2 gene is constructed.

English

0208|AGRIS 0401|AL-Wheat Program|R02CIMPU

Juan Carlos Mendieta

CIMMYT Publications Collection


International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org