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A DNA Microarray-based assay to detect dual infection with two dengue virus serotypes

By: Contributor(s): Material type: ArticleArticleLanguage: En Publication details: 2014ISSN:
  • 1424-8220
Subject(s): Online resources: In: Sensors v. 14, no. 5, p. 7580-7601Summary: Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples.
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Article CIMMYT Knowledge Center: John Woolston Library CIMMYT Staff Publications Collection Available
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Peer-review: Yes - Open Access: Yes | http://www.mdpi.com/journal/sensors

Peer review

Open Access

Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples.

Genetic Resources Program

English

CIMMYT Informa 2015

INT3239

CIMMYT Staff Publications Collection


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