Mapping QTLs for grain yellow pigment content in the cultivated durum wheat germplasm
Material type:
TextPublication details: 2009Description: p. 191Summary: Grain yellow pigment content (GYP, xanthophyll pigments) is an important quality trait in durum wheat, where a bright yellow colour is a standard quality pre-requisite for semolina and pasta products. Although GYP is a quantitative trait with a relatively high heritability, a considerable portion of the cultivated germplasm still has GYP lower than the market requirements. High GYP germplasm sources have been identified and are being actively exploited in breeding programs. Thus, molecular markers tightly linked to the major GYP genes/QTLs are needed for MAS. We studied GYP in the cultivated durum wheat with a comprehensive genetic study including three RIL populations: (1) KS: Kofa (high GYP) × Svevo (medium), with 249 RILs, (2) CL: Colosseo (loow) × Lloyd (high), with 176 RILs, (3) MC: Meridiano (medium-high) × Claudio (medium-low), with 181 RILs, and a germplasm collection (Panel) of 189 cultivated durum accessions bred for Mediterranean areas plus a further panel of NorthAmerican durums. These materials were grown in Italy and in other Mediterranean countries over several years and locations and whole grain yellow index was determined as b* (Minolta b-value). Linkage maps for the three RIL populations were obtained with SSR and DArT markers from the Triticarte Durum Array v 2.0 (Maccaferri et al., 2008; Mantovani et al., 2008; Maccaferri et al., unpublished), with an average intermarker distance of 5 cM. The Panel was genotyped with 180 SSRs of known map position (as from the RIL linkage maps) and with 900 DArT markers. Major QTLs from one or two populations (average R2 > 5% using mean data) were found in the following chr. regions: chr. 1AS (CL), chr. 2BL (KS), chr. 3BS (KS), chr. 4AL (KS), chr. 4BL (KS and CL), chr. 5A centromeric (5Ac, MC), 5BL (KS, CL, MC), chr. 6Ac (KS and CL), chr. 7Ac and 7Bc (KS and CL, homeologous positions), chr. 7AL and 7BL (KS and MC, homeologous distal position). These QTLs were validated via association mapping in the Panel and the effects of the increasing alleles were estimated. The detailed results of the QTL and association analysis will be presented and discussed.
| Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|
| Conference proceedings | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | CIS-5826 (Browse shelf(Opens below)) | Available |
Abstract only
Grain yellow pigment content (GYP, xanthophyll pigments) is an important quality trait in durum wheat, where a bright yellow colour is a standard quality pre-requisite for semolina and pasta products. Although GYP is a quantitative trait with a relatively high heritability, a considerable portion of the cultivated germplasm still has GYP lower than the market requirements. High GYP germplasm sources have been identified and are being actively exploited in breeding programs. Thus, molecular markers tightly linked to the major GYP genes/QTLs are needed for MAS. We studied GYP in the cultivated durum wheat with a comprehensive genetic study including three RIL populations: (1) KS: Kofa (high GYP) × Svevo (medium), with 249 RILs, (2) CL: Colosseo (loow) × Lloyd (high), with 176 RILs, (3) MC: Meridiano (medium-high) × Claudio (medium-low), with 181 RILs, and a germplasm collection (Panel) of 189 cultivated durum accessions bred for Mediterranean areas plus a further panel of NorthAmerican durums. These materials were grown in Italy and in other Mediterranean countries over several years and locations and whole grain yellow index was determined as b* (Minolta b-value). Linkage maps for the three RIL populations were obtained with SSR and DArT markers from the Triticarte Durum Array v 2.0 (Maccaferri et al., 2008; Mantovani et al., 2008; Maccaferri et al., unpublished), with an average intermarker distance of 5 cM. The Panel was genotyped with 180 SSRs of known map position (as from the RIL linkage maps) and with 900 DArT markers. Major QTLs from one or two populations (average R2 > 5% using mean data) were found in the following chr. regions: chr. 1AS (CL), chr. 2BL (KS), chr. 3BS (KS), chr. 4AL (KS), chr. 4BL (KS and CL), chr. 5A centromeric (5Ac, MC), 5BL (KS, CL, MC), chr. 6Ac (KS and CL), chr. 7Ac and 7Bc (KS and CL, homeologous positions), chr. 7AL and 7BL (KS and MC, homeologous distal position). These QTLs were validated via association mapping in the Panel and the effects of the increasing alleles were estimated. The detailed results of the QTL and association analysis will be presented and discussed.
Global Wheat Program
English
Lucia Segura
INT2585
CIMMYT Staff Publications Collection
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