Genetic diversity of a DREB-related gene in durum wheat and its wild relatives
Material type:
TextPublication details: 2010Description: p. 206Summary: The genetic diversity of various accessions of durum wheat, Aegilops speltoides, Triticum urartu and different Aegilops species has been investigated by analysing the structure of the DRF1 gene, which belongs to DREB-family and is involved in the abiotic stress response. We have cloned and fully characterized the DRF1 gene in about 100 durum accessions and more than 200 wild ancestor accessions. The alternative splicing mechanism that characterizes the DRF1 gene expression in wheat was also observed in its wild relatives. SNP, SSR and insertion/deletion were detected and analysed by various bioinformatic tools in all the obtained sequences, in view of gaining more knowledge about the intra- and inter-specific variability of this gene, of its pseudogenes and of its homeologous copies. Furthermore, we have identified a novel transposon encompassing intron1-intron3, thus including exon2 and exon3. Due to its characteristics, it was used as a tool for investigating the genesis of the DREB-genes. An interactive database was built to store, access and compare all the derived information.
| Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|
| Conference proceedings | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | CIS-6176 (Browse shelf(Opens below)) | Available |
Abstract only
The genetic diversity of various accessions of durum wheat, Aegilops speltoides, Triticum urartu and different Aegilops species has been investigated by analysing the structure of the DRF1 gene, which belongs to DREB-family and is involved in the abiotic stress response. We have cloned and fully characterized the DRF1 gene in about 100 durum accessions and more than 200 wild ancestor accessions. The alternative splicing mechanism that characterizes the DRF1 gene expression in wheat was also observed in its wild relatives. SNP, SSR and insertion/deletion were detected and analysed by various bioinformatic tools in all the obtained sequences, in view of gaining more knowledge about the intra- and inter-specific variability of this gene, of its pseudogenes and of its homeologous copies. Furthermore, we have identified a novel transposon encompassing intron1-intron3, thus including exon2 and exon3. Due to its characteristics, it was used as a tool for investigating the genesis of the DREB-genes. An interactive database was built to store, access and compare all the derived information.
Global Wheat Program
English
INT2585
CIMMYT Staff Publications Collection
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